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  10. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid
    #185289
    Purpose
    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation
    Depositor
    Pamela Silver
    Article
    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1
    Insert
    iLID::eGFP::FTH1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185289-plasmid-map-sequence-id-363896

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  15. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

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Showing: 10181 - 10200 of 22786 results