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pv6_CBX7_Chromo-W35A_TAF3_Phd

Plasmid

#179401

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_Chromo(W35A) - TAF3_Phd

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Chromodomain W35A

Promoter

CAGGS

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pv7_CBX1_2xChromo_noEGFP

Plasmid

#179395

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX1_2xChromo_noEGFP

Use

Tags

AviTag

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MBD1_MBD-R22A

Plasmid

#179405

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MBD1_MBD(R22A)

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R22A

Promoter

CAGGS

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMAL-p2x-LanCL2

Plasmid

#154192

Purpose

To express MBP-LanCL2 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Maltose binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSN554

Plasmid

#177361

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper, mScarlet and StrepII tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Use

Tags

Leucine zipper, StrepII tag, and mScarlet

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSN687

Plasmid

#177363

Purpose

Expresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Use

Tags

His tag and Leucine zipper

Expression

Bacterial

Mutation

changed Proline 305 to Leucine

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst1-3xHA

Plasmid

#183540

Purpose

Express Dcst1-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D

Plasmid

#115193

Purpose

Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

p.S291D

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S293D

Plasmid

#115194

Purpose

Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S293D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

p.S293D

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S291D/S293D

Plasmid

#115195

Purpose

Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S291D/S293D (PDHA2 Human)

Use

Lentiviral

Tags

Expression

Mutation

p.S291D/S293D

Promoter

EF1alpha

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_2

Plasmid

#185023

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_1

Plasmid

#185022

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_2

Plasmid

#185021

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_1

Plasmid

#185020

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2

Plasmid

#185019

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_1

Plasmid

#185018

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_SpDHN1 (pBS0799)

Plasmid

#185261

Purpose

For the mammalian expression of the grass (Stipa purpurea) protein DHN_SpDHN1. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_SpDHN1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_ShDHN (pBS0797)

Plasmid

#185260

Purpose

For the mammalian expression of the tomato plant protein DHN_ShDHN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_ShDHN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(DM)-Fc-IG

Plasmid

#183725

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q A108V mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Expression

Mutation

R47Q and A108V

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-GFP11-RhoA

Plasmid

#181860

Purpose

Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-RhoA (RHOA Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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