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Plasmid#102535PurposeTemplate for synthesis of Minos transposase mRNADepositorInsertMinos transposase mRNA
UseTagsExpressionMutationPromoterAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-134
Plasmid#185925PurposeHimar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein geneDepositorInsertmOrange2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHSS6hsILMi20
Plasmid#102536PurposeHelper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)DepositorInsertMinos transposase
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_QRFV_Hyp
Plasmid#207302PurposeMammalian expression of Quaranfil quaranjavirus Hypothetical proteinDepositorInsertQRFV_Hyp
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA CO Tol2 TPase
Plasmid#133032PurposeExpression vector for production of codon optimized Tol2 transposaseDepositorInsertCodon optimized Tol2 Transposase
UseIn vitro expressionTagsExpressionMutationCodon OptimizedPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantMutationPromoter2x35SAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
wg186Fluc
Plasmid#177436PurposeIn vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.DepositorInsertFirefly luciferase
UseLuciferase; For in vitro transcriptionTagsNoneExpressionMutationPromoterT7Available sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TfR-OVA
Plasmid#64600PurposeExpression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentationDepositorInsertOvalbumin
UseTagsTransmembrane domain of Transferrin Receptor (TfR…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_hACE2_HygR
Plasmid#155296PurposeLentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistanceDepositorInserthACE2 (ACE2 Human)
UseLentiviralTagsC9ExpressionMutationPromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKG-GW2-MaUPPS-B
Plasmid#203175PurposeYeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertMA_4402
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKG-GW1-MaUPPS-A
Plasmid#203174PurposeYeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferaseDepositorInsertuppS
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2-AA007
Plasmid#185750PurposeVector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
UseTagsmembrane localization tag Lti6bExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
L2-AA012
Plasmid#185751PurposeVector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
UseTagsmembrane localization tag Lti6bExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
EYFP-hDF-deltaTM-pCMV-9
Plasmid#72586Purposehuman dysferlin no transmembrane domain tagged with YFPDepositorInsertdysferlin (DYSF Human)
UseTagsEYFPExpressionMammalianMutationtransmembrane domain deletedPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CDK9 HA (P#635)
Plasmid#14640DepositorInsertcdk9 (CDK9 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…TagsExpressionPlantMutationPromoterE12-omegaAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHis6-hDF-deltaTM-pCMV-9
Plasmid#72587Purposehuman dysferlin no transmembrane domain tagged with his tagDepositorInsertdysferlin (DYSF Human)
UseTagsHisExpressionMammalianMutationtransmembrane domain deletedPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only