Showing: 1001 - 1020 of 15987 results
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Plasmid#185765PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-CTERM-GFP
Plasmid#185770PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
PGK-DTAG-NTERM-GFP
Plasmid#185775PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
SspB-HaloTag-DHPH-TIAM1
Plasmid#176114PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FKBP12-CB2mutx2-mTagBFP2-HAx2
Plasmid#207147PurposeTandem mutant calcium-buffer with rapalog dimerizerDepositorInsertCalbindin-2 (CALB2 Human)
UseTagsFKBP12, HA, and mTagBFP2ExpressionMammalianMutationE40Q, E87Q, E131Q, E175Q, E219QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-DNAJC5 (L115R)-HaloTag
Plasmid#205711PurposeMammalian expression of DNAJC5 (L115R)-HaloTagDepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsHaloTagExpressionMammalianMutationL115RPromoterAvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H3C2
Plasmid#207783PurposeDonor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorArticleInsertH3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorArticleInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
AID-mTagBFP2-loxP_myo2_neoR-loxP
Plasmid#194055PurposeDual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genomeDepositorInsertIAA17 (AXR3 Mustard Weed)
UseTagscodon optimized mTagBFP2ExpressionWormMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-TagRFP-T-IFT88
Plasmid#61684PurposeMammalian expression of IFT88 fused with N terminal TagRFP.T tagDepositorInsertIFT88 (Ift88 Mouse)
UseTagsTagRFP.TExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
csd6_17-330/pET28b(+) N-tag
Plasmid#71353Purposecsd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)DepositorInsertHP0518 (HP0518 )
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_E110A/pET28b(+) N-tag
Plasmid#71354Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:E110ADepositorInsertHP0518 (HP0518 changed Glutamic acid 110 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y132A/pET28b(+) N-tag
Plasmid#71355Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y132ADepositorInsertHP0518 (HP0518 changed Tyrosine 132 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y133A/pET28b(+) N-tag
Plasmid#71356Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y133ADepositorInsertHP0518 (HP0518 changed Tyrosine 133 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_H155A/pET28b(+) N-tag
Plasmid#71357Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H155ADepositorInsertHP0518 (HP0518 changed Histidine 155 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_W158A/pET28b(+) N-tag
Plasmid#71358Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:W158ADepositorInsertHP0518 (HP0518 changed Tryptophan 158 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_H160A/pET28b(+) N-tag
Plasmid#71359Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H160ADepositorInsertHP0518 (HP0518 changed Histidine 160 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_C176A/pET28b(+) N-tag
Plasmid#71360Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:C176ADepositorInsertHP0518 (HP0518 changed Cysteine 176 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagExpressionMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …AvailabilityAcademic Institutions and Nonprofits only -
pPW3759 : CMVd3-HsIRE1deltaLD-HaloTag
Plasmid#185681PurposeLow-level transient expression of delta-lumenal domain HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsHaloTagExpressionMammalianMutationDeletion of lumenal domainPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1001 - 1020 of 15987 results