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Showing: 1001 - 1020 of 1167 results

  2. pDD285

    Plasmid
    #66826
    Purpose
    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.
    Insert
    mKate2-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans codon-optimized mKate2
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  3. pOCC202

    Plasmid
    #118880
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    mKate2, HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Availability
    Academic Institutions and Nonprofits only
    118880-plasmid-map-sequence-id-232243

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  4. pOCC204

    Plasmid
    #118894
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C and C-terminal mKate2, and HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
    Use
    Tags
    MBP, cleavable with 3C protease and mKate2, HIS6,…
    Expression
    Insect
    Mutation
    Promoter
    polH
    Availability
    Academic Institutions and Nonprofits only
    118894-plasmid-map-sequence-id-232313

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  5. LSB-hsa-miR-92a-1-5p

    Plasmid
    #103750
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-92a-1-5p target (MIR92A1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Availability
    Academic Institutions and Nonprofits only
    103750-plasmid-map-sequence-id-196548

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  8. pLenti-Synapsin-pHoenix-WPRE

    Plasmid
    #70111
    Purpose
    lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    pHoenix (Syp Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
    Use
    Lentiviral
    Tags
    Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…
    Expression
    Mutation
    Promoter
    Synapsin
    Availability
    Academic Institutions and Nonprofits only
    70111-plasmid-map-sequence-id-177119

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  10. pJW1586

    Plasmid
    #121057
    Purpose
    mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2-T^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized mKate2, and au…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121057-plasmid-map-sequence-id-235972

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  11. pJW1595

    Plasmid
    #121062
    Purpose
    mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mKate2^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121062-plasmid-map-sequence-id-235983

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  12. pWZ194

    Plasmid
    #163640
    Purpose
    rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Nematode, Synthetic)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Availability
    Academic Institutions and Nonprofits only
    163640-plasmid-map-sequence-id-324064

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  13. pAWK61

    Plasmid
    #163642
    Purpose
    rps-27>DHB::GFP-P2A-H2B::2xmKate2 expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I
    Depositor
    David Matus
    Article
    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1
    Insert
    DHB::GFP-P2A-H2B::2xmKate2::3xHA (his-58 Nematode, Human)
    Use
    CRISPR
    Tags
    2xmKate2 and GFP
    Expression
    Worm
    Mutation
    Promoter
    rps-27
    Availability
    Academic Institutions and Nonprofits only
    163642-plasmid-map-sequence-id-362086

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  14. FRP266

    Plasmid
    #159304
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mKate2 under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159304-plasmid-map-sequence-id-311199

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  15. pTD56

    Plasmid
    #172895
    Purpose
    mKate2^SEC^AID* vector with ccdB markers for cloning homology arms
    Depositor
    David Reiner
    Insert
    mKate2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    172895-plasmid-map-sequence-id-353627

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  16. pTD72

    Plasmid
    #172896
    Purpose
    mKate2^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    David Reiner
    Insert
    mKate2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    172896-plasmid-map-sequence-id-353630

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  17. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    PTEN exon 5 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

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  18. px330_P53 exon 8 gRNA

    Plasmid
    #68349
    Purpose
    px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 8 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68349-plasmid-map-sequence-id-119621

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  19. px330_SMAD exon 2 gRNA

    Plasmid
    #68350
    Purpose
    px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    SMAD exon 2 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68350-plasmid-map-sequence-id-119622

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  20. px330_P53 exon 7 gRNA

    Plasmid
    #68351
    Purpose
    px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Insert
    P53 exon 7 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    68351-plasmid-map-sequence-id-119623

    Enlarge

Showing: 1001 - 1020 of 1167 results