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Plasmid#200910Purpose3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsert3XHA_den1FnCas9_KRAB_eGFP
UseCRISPRTags3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFPExpressionMammalianMutationChanged Glutamic Acid to Arginine at 1369 positionPromoterCBHAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBacMam5_N_Flag_His8_eGFP_3C
Plasmid#178816PurposeBaculovirus gene transfer into Mammalian cells to product recombinant proteins.DepositorTypeEmpty backboneUseTagsFlag, His8, eGFP, 3CExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMax_GFP_C289T
Plasmid#177826PurposeSite-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).DepositorInsertGFP_C289T
UseTagsExpressionMammalianMutationC289TPromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
h-Plekhm1_EGFP
Plasmid#73836PurposeExpress human Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ASPSCR1_TFE3
Plasmid#205776PurposeExpress mEGFP-tagged fusion protein, ASPSCR1_TFE3 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGFP
Plasmid#64904Purpose"Fluorescent reporter containing no disulfide bonds/Selenocystamine folding"DepositorInsertGreen fluorescent protein
UsePsba chlamydomonas reinhardtii vectorTagsExpressionMutationPromoterpsbAAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
punc503GFP
Plasmid#64021Purpose503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expressionDepositorInsertunc-45b: GFP (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA.SFFV.GFP
Plasmid#169938PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX3_FOXO1
Plasmid#205875PurposeExpress mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT480GFP
Plasmid#96905Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15DepositorInsertEGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-H4-23
Plasmid#56463PurposeLocalization: Nucleus/Histones, Excitation: 488, Emission: 507DepositorInsertH4 (HIST1H4J Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP_rescue_construct
Plasmid#190641PurposePuromycin-selectable expression of GFP in Drosophila S2 cellsDepositorInsertEGFP
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZE27GFP5
Plasmid#75458PurposePlasmid for constitutive expression of gfp-pdt5DepositorInsertgfp-pdt#5
UseTagsExpressionBacterialMutationPromoterplacIq promoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_XPR2_hrGFP
Plasmid#84614PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGFP150K
Plasmid#193313PurposepAS control plasmid for sfGFP expressionDepositorInsertsuper folder GFP
UseTagsExpressionMammalianMutation150K in sfGFPPromoterEF1Available sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_A08_hrGFP
Plasmid#84615PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locusDepositorInserthrGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_YAP1_MAMLD1
Plasmid#205955PurposeExpress mEGFP-tagged fusion protein, YAP1_MAMLD1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_sensor_GFP
Plasmid#139463PurposeT7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporterDepositorInsertSNIPR_GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only