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Plasmid#207091PurposepX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.DepositorInsertGGGGAGCAGATGGACCCTAC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDC1 N-terminal sgRNA
Plasmid#207090PurposepX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.DepositorInsertGTATCCTTCCCAGATCATGG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
SHLD3 KO sgRNA #2
Plasmid#207106PurposepX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..DepositorInsertCTGAAGGAACAGACTAATTC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
53BP1 KO sgRNA
Plasmid#207104PurposepX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.DepositorInsertAGATTCTCAGCCTGAAAGCC
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTST-EGFP-GPIBY55
Plasmid#213706PurposeExpress the affinity sorting tag TST-EGFP- GPIBY55.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTST-EGFP-GPICEAM7
Plasmid#213705PurposeExpress the affinity sorting tag TST-EGFP- GPICEAM7.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTST-EGFP-GPIDAF
Plasmid#213704PurposeExpress the affinity sorting tag TST-EGFP- GPIDAF.DepositorInsertEGFP
UseTagsTwin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TREER
Plasmid#208321PurposeBacterial expression of the His-tagged peptide TREER.DepositorInsertTREER
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b-TinyERRE
Plasmid#208320PurposeBacterial expression of the His-tagged peptide TinyERRE.DepositorInsertTinyERRE
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2_pJEx
Plasmid#213908PurposeBarcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR BsmAIM
Plasmid#206206PurposeConstitutive expression of BsmAIM methyltransferaseDepositorInsertBsamAIM
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-EGFP-Cre
Plasmid#207132PurposeLentiviral vector to express EGFP-CreDepositorInsertEGFP-Cre
UseLentiviralTagsExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PuRRRE
Plasmid#208323PurposeBacterial expression of the His-tagged peptide PuRRRE.DepositorInsertPuRRRE
UseTagsDBCO-Tag, His-TagExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-NLS-ZapCV2
Plasmid#203664PurposeExpresses zinc sensor ZapCV2 in the nucleus of mammalian cellsDepositorInsertZapCV2 Zinc sensor
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT346
Plasmid#204505Purpose5S rDNA used as a probe for chromosome FISH in nematodeDepositorInsert5S rDNA (C. elegans)
UseOtherTagsExpressionMutationPromoterT7Available sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMAH-pnRFP
Plasmid#201679PurposeExpresses pnRFP in mammalian cellsDepositorInsertpnRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-mRFP
Plasmid#170982PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-fullef1a-mutFAKSPARK2
Plasmid#195266Purposeexpress Tyr to Phe mutant FAKSPARK2 in a lenti vectorDepositorInsertTyr to Phe mutant FAKSPARK2 sensor
UseLentiviralTagsExpressionMammalianMutationPromoteref1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBP60g-NLS-3xmCitrine
Plasmid#192521PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertCALMODULIN BINDING PROTEIN 60g
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterCBP60gAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 LvWSCP-EGFP
Plasmid#196480PurposeMammalian expression of WSCP-EGFPDepositorInsertLvWSCP-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-SpDcp2opt_1-242_AD
Plasmid#148547PurposeBacterial Expression of SpDcp2opt_1-242DepositorInsertSpDcp2opt_1-242 (dcp2 Fission Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-SpDCP1_X
Plasmid#148003PurposeBacterial Expression of SpDCP1DepositorInsertSpDCP1 (dcp1 Fission Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-CtDCP2_1-267_X
Plasmid#148004PurposeBacterial Expression of CtDCP2_1-267DepositorInsertCtDCP2_1-267
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A-N-term_F
Plasmid#146300PurposeMammalian Expression of HsTNRC6A-NtermDepositorInsertHsTNRC6A-Nterm (TNRC6A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1-delMI-MII_F
Plasmid#146304PurposeMammalian Expression of HsTNRC6Biso1-delMI-MIIDepositorInsertHsTNRC6Biso1-delMI-MII (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C-delAGOhook_F
Plasmid#146307PurposeMammalian Expression of HsTNRC6C-delAGOhookDepositorInsertHsTNRC6C-delAGOhook (TNRC6C Human)
UseTagsExpressionMammalianMutation1 silent mutations compared to the sequence of Hs…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C-delMI_F
Plasmid#146308PurposeMammalian Expression of HsTNRC6C-delMIDepositorInsertHsTNRC6C-delMI (TNRC6C Human)
UseTagsExpressionMammalianMutation2 silent mutations compared to the sequence of Hs…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C-delMII_F
Plasmid#146310PurposeMammalian Expression of HsTNRC6C-delMIIDepositorInsertHsTNRC6C-delMII (TNRC6C Human)
UseTagsExpressionMammalianMutation2 silent mutations compared to the sequence of Hs…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C-delMI-MII_F
Plasmid#146311PurposeMammalian Expression of HsTNRC6C-delMI-MIIDepositorInsertHsTNRC6C-delMI-MII (TNRC6C Human)
UseTagsExpressionMammalianMutation2 silent mutations compared to the sequence of Hs…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-RLuc-6xbsMS2-8xlet7_AC
Plasmid#148433PurposeMammalian Expression of 6xbsMS2-8xlet7DepositorInsert6xbsMS2-8xlet7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-TISU-RLuc-6xbsMS2_AC
Plasmid#148434PurposeMammalian Expression of 6xbsMS2DepositorInsert6xbsMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-TISU-RLuc-6xbsMS2-8xlet7_AC
Plasmid#148435PurposeMammalian Expression of 6xbsMS2-8xlet7DepositorInsert6xbsMS2-8xlet7
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-TISU-RLuc-6xbsMS2-random_AC
Plasmid#148437PurposeMammalian Expression of 6xbsMS2-randomDepositorInsert6xbsMS2-random
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-no5UTR-lambdaN-HA-GST-V5His6_AC
Plasmid#148405PurposeInsect Expression vectorDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-no5UTR-Fluc-5BoxB_AC
Plasmid#148408PurposeInsect Expression of 5BoxBDepositorInsert5BoxB
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP
Plasmid#185679PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.DepositorInsertERmembrane-2xHaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pnEA-NpG-SpDcp1opt_AD
Plasmid#148538PurposeBacterial Expression of SpDcp1optDepositorInsertSpDcp1opt (dcp1 Fission Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-TRE3G-Nluc
Plasmid#192659PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-NlucDepositorInsertNluc
UseLuciferaseTagsExpressionMammalianMutationPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-TRE3G-TdTomato
Plasmid#192658PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomatoDepositorInsertTdTomato
UseTagsExpressionMammalianMutationPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-miniCMV-Nluc
Plasmid#192657PurposeA plasmid encoding miniCMV sgRNA and miniCMV-promoter-NlucDepositorInsertNluc
UseLuciferaseTagsExpressionMammalianMutationPromoterU6, miniCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-VP64_Blast
Plasmid#192655Purpose3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance markerDepositorInsertscFv-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_SKL
Plasmid#182007PurposeCMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell linesDepositorInsertSTf_SKL
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf
Plasmid#182000PurposeCMV overexpression of SNAP-tag fast in mammalian cell linesDepositorInsertSTf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_NES_STf
Plasmid#182001PurposeCMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell linesDepositorInsertNES-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179010PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179009PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only