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Plasmid#60513PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only
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pet30b-7M SrtA
Plasmid#51141Purposeexpression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 7M
UseTags6x HisExpressionBacterialMutationP94R, E105K, E108Q, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
phND2-N174
Plasmid#31822DepositorInsertNeuroD2 (NEUROD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-puro
Plasmid#125839Purposemodifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
LXRalpha_pcDNA3.1
Plasmid#177621PurposeFull-length human LXR alpha cDNA sequence in pcDNA3.1+DepositorInsertLXR alpha (NR1H3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-ssRFP-GFP-KDEL
Plasmid#128257PurposeFluorescence reporter to monitor ER-phagy activityDepositorInsertssRFPGFPKDEL
UseLentiviralTagsRFPGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-Sumo2 WT
Plasmid#48967PurposeExpresses HA SUMO2 Wild Type in mammalian cellsDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHAExpressionMammalianMutationPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP6s
Plasmid#40753PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertGCaMP6s
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterNAAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
STAT3-GFP
Plasmid#110495Purposereporter of STAT3 signaling activityDepositorInsert4 repeats of STAT3 binding site M67 at GFP promoter region
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLXSN18E6E7
Plasmid#53459PurposeThe plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.DepositorInsertHPV18E6E7
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMMP-BiP-IRES-mRFP
Plasmid#36975DepositorInsertBiP (HSPA5 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-X
Plasmid#81071PurposeEmpty lentiviral backbone with the MNDU3 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDU3Available sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Blast
Plasmid#85973PurposeTet/Dox inducible shRNA lentivirus with blasticidin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IQGAP1
Plasmid#30112DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagstandem EGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-parkin WT
Plasmid#45875DepositorInsertParkin (PRKN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE_GFP-GluR1
Plasmid#34857DepositorInsertGluR1 (Gria1 Mouse)
UseMouse TargetingTagsGFPExpressionMammalianMutationPromoterTREAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G
Plasmid#194457PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1MCS-2
Plasmid#85168PurposeMobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionBacterialMutationPromoterConstitutive (derepressed P-lac)Available sinceNov. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lentiviral-S-tdTomato
Plasmid#112579PurposeExpressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vectorDepositorInsertNLS-tdTomato-NES
UseLentiviralTagsnuclear export sequence and nuclear localization …ExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry
Plasmid#207668PurposeTo express fluorescently labeled MS2 coat binding protein (MCP)DepositorInsertMCP-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-jRGECO1a
Plasmid#61563PurposeRed fluorescent calcium sensor proteinDepositorInsertNES-jRGECO1a
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-BSR-GFP11
Plasmid#68716PurposeSplit GFP assayDepositorInsertbsr
UseRetroviralTagsFLAG and GFP11ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_dCas9_KRAB
Plasmid#126030PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusionDepositorInsertsdCas9 fusion with KRAB
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOUP
Plasmid#165419PurposeThe pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in AgrobacteriumInsertRep A
UseHelper plasmid required for replication for all p…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only