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Plasmid#78334PurposeDoxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tagDepositorInsertMYF5 (MYF5 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-Cbx7-delta-(CD -ATL)
Plasmid#82524PurposeLentivirus vector. Expresses mutant Halotag-Cbx7 fusion proteins in mammalian cells. Cbx7 deletion of amino acids 1–83.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationdeletion of amino acids 1–83PromoterCMVAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGH312_wtGFP Q80H
Plasmid#85409Purposelentiviral expression vector for wtGFP Q80HDepositorInsertwtGFP Q80H
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGH314_wtGFP S65T, Q80H
Plasmid#85407Purposelentiviral expression vector for wtGFP S65T-Q80HDepositorInsertwtGFP S65T, Q80H
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GRK5 gRNA (BRDN0001149126)
Plasmid#76271Purpose3rd generation lentiviral gRNA plasmid targeting human GRK5DepositorInsertGRK5 (GRK5 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch
Plasmid#10881DepositorInsertH1-shSOD1mismatch (SOD1 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
TRIM27 gRNA (BRDN0001149189)
Plasmid#77514Purpose3rd generation lentiviral gRNA plasmid targeting human TRIM27DepositorInsertTRIM27 (TRIM27 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRIM27 gRNA (BRDN0001149307)
Plasmid#77515Purpose3rd generation lentiviral gRNA plasmid targeting human TRIM27DepositorInsertTRIM27 (TRIM27 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV-EF1aGFP2AKAT7(E508Q)-W
Plasmid#159293PurposeLentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoterDepositorInsertKAT7 E508Q
UseLentiviralTagsExpressionMammalianMutationCRISPR sites mutated (gRNA ID. 5 and A10) and E50…Promoterhuman EF1a promoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shLacZ-1735
Plasmid#115454PurposeConstitutive lentiviral expressionDepositorInsertshLacZ-1735
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer
Plasmid#86195PurposeU6 based expression of N meningitidis sgRNADepositorInsertnmCas9 sgRNA cloning cassete
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Barcode backbone
Plasmid#158665PurposeThis plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combinationDepositorTypeEmpty backboneUseLentiviralTagshcREDExpressionMammalianMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GNE gRNA (BRDN0001149214)
Plasmid#77831Purpose3rd generation lentiviral gRNA plasmid targeting human GNEDepositorInsertGNE (GNE Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRIP13 H3.2 gRNA
Plasmid#90920Purpose3rd generation lentiviral gRNA plasmid targeting human TRIP13DepositorInsertTRIP13 (Guide Designation H3.2)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
SRC gRNA (BRDN0001146185)
Plasmid#75651Purpose3rd generation lentiviral gRNA plasmid targeting human SRCDepositorInsertSRC (SRC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K1 gRNA (BRDN0001145794)
Plasmid#76210Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K1DepositorInsertMAP2K1 (MAP2K1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K1 gRNA (BRDN0001147078)
Plasmid#76211Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K1DepositorInsertMAP2K1 (MAP2K1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K1 gRNA (BRDN0001147218)
Plasmid#76212Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K1DepositorInsertMAP2K1 (MAP2K1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only