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Plasmid#177102PurposeExpresses Ent5-HaloTag in yeast cellsDepositorInsertENT5 (ENT5 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXS-sfGFP
Plasmid#117386PurposepGEN-mcs with a modular sfGFP expression scaffoldDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtacAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7deltaE3
Plasmid#175748PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.DepositorInsertAdenovirus 5 genomic region 25043-35938
UseAdenoviral and Synthetic BiologyTagsExpressionMutationAdenovirus sequences 27859-30803 replaced with Ba…PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Double UP mClover3 to mScarlet
Plasmid#120262PurposeNatively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.DepositorInsertmClover3/mScarletP2A-mcs
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarletPromoterCAGAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
P-miR-145 (pTransluc-miR-145-1)
Plasmid#21496DepositorInsert1.5kb 5' of miR-145 (MIR145 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBB14
Plasmid#18657DepositorInsertUs9-EGFP gene fusion
UseTagsEGFPExpressionMammalianMutationFirst 106 amino acids of Us9 are intact. The stop…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJEC625
Plasmid#174368PurposeGolden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
258/-2.5CAT
Plasmid#25431DepositorInsertMyo D Promoter/Enhancer (MYOD1 Human)
UseReporter constructTagsCATExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4-Myc-HisB-Sirt3
Plasmid#24918DepositorInsertSirt3 (SIRT3 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pAAVS1-delta8.2R
Plasmid#89706PurposeDonor plasmid for HIV-1 env- integration into human AAVS1 locusDepositorInsertAAVS1-delta8.2R
UseUnspecifiedTagsExpressionMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-RASSF1A
Plasmid#1980DepositorInsertRASSF1A (Rassf1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-15
Plasmid#119994PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7026
Plasmid#128811PurposeFluoride riboswitch-regulated 3-way junction dimeric broccoliDepositorInsertFluoride riboswitch-regulated 3-way junction dimeric broccoli
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiRc3_shNT
Plasmid#81067PurposeGateway entry vector encoding non-targeting shRNADepositorInsertnon-targeting shRNA
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK_Snai2_enh_13_Citrine
Plasmid#130574Purposeenhancer reporterDepositorInsertSnai2_enh_13
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SMAD2_HAe2_Bsd
Plasmid#167166PurposeBlasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.DepositorInsertRepair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
UseRepair template for gene editing in human cellsTagsblasticidinExpressionMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-USP44-HIS
Plasmid#43894DepositorInsertUSP44 (USP44 Human)
UseTagsHISExpressionMammalianMutationPromoterAvailable sinceApril 12, 2013AvailabilityAcademic Institutions and Nonprofits only