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Plasmid#133073PurposePlasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tagDepositorInsertLis1 (PAFAH1B1 Human)
UseTags8x His, Linker-TEV-linker_TEV, SNAPf, and ZZExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[3]-C (LEU2)
Plasmid#177796PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[3]ExpressionYeastMutationPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-BAX-201
Plasmid#117332PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR BAX-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-EGFP-PolB-Hygro
Plasmid#176056PurposeEGFP fused to the N-terminus of PolB & a hygromycin resistance cassetteDepositorInsertPolB (POLB Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
[pSK496] pLJC5 HA-Depdc5(AB)
Plasmid#109346PurposeLenti-viral expression of HA-tagged Depdc5 mutant ABDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsHAExpressionMutationDIYGD(185-189)GSGSG;EQPLH(371-375)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorInsertC9ORF80 (INIP Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-DNMT3a ts3
Plasmid#115867PurposeDNMT3a knockdownDepositorInsertDNMT3a shRNA (DNMT3A Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR
Plasmid#75136PurposeRetroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR
Plasmid#75135PurposeRetroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp7-HF
Plasmid#157690Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoterDepositorInsertNsp7-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nprot
Plasmid#157729Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nprot (N SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA1 Flag ATXN1[85Q]-S776A
Plasmid#33240DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsflagExpressionMammalianMutationinsert contains a stretch of 85 Glutamines and no…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp9
Plasmid#157704Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp9 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT-TO-FH-Nsp10
Plasmid#157707Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp10 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6P-1-INTS3-N
Plasmid#128416PurposeExpress INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
UseTagsGSTExpressionBacterialMutationN-terminal region (1–513 aa)Promotertac promoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
MLS-mir-17-92-Mut92
Plasmid#64094Purposeexpresses a mutated version of mir-17-92 (mutation of miR-92 seed)DepositorInsertmiR-17-92 (MIR17HG Human)
UseTagsExpressionMammalianMutationmutation of miR-92 seed sequencePromoterMSCVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMyc-C1-Cep123 (CCDC123/Cep89)
Plasmid#69836PurposeThis plasmid encodes Cep123 (CCDC123/Cep89) isoform 1 with an N-ter Myc-tagDepositorInsertCep123 (CEP89 Human)
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a
Plasmid#79592PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-Cep123-C2 domain
Plasmid#73329PurposeEncodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFPDepositorInsertCep123 (CEP89 Human)
UseTagsEGFP tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only