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Showing: 10141 - 10160 of 52000 results

  2. pYPQ262B-ABE8e

    Plasmid
    #161524
    Purpose
    Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    TadA8e-zSpRYCas9(D10A)
    Use
    CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161524-plasmid-map-sequence-id-325557

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  4. pJW1584

    Plasmid
    #121055
    Purpose
    YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    YPET^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized YPET, and auxi…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121055-plasmid-map-sequence-id-235991

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  10. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Promoter
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  13. pGLOW39

    Plasmid
    #173068
    Purpose
    mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms
    Depositor
    Ryan Doonan
    Article
    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791
    Insert
    mScarlet-I-C1^SEC^3xMyc
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    173068-plasmid-map-sequence-id-345944

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  14. AURKA_HUMAN_D0

    Plasmid
    #79739
    Purpose
    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    AURKA (AURKA Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pYPQ266

    Plasmid
    #164713
    Purpose
    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    zCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164713-plasmid-map-sequence-id-325387

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  19. pYPQ269E2

    Plasmid
    #164721
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
    Use
    CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164721-plasmid-map-sequence-id-325393

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Showing: 10141 - 10160 of 52000 results