Skip to main content
Addgene
Showing: 10141 - 10160 of 52614 results

  1. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

    Enlarge

  2. pIC162

    Plasmid
    #63070
    Purpose
    Retroviral expression of EGFP-TEV-S-tag Nuf2 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    Nuf2 (NUF2 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pREP4xN_ccdB

    Plasmid
    #79022
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79022-plasmid-map-sequence-id-147779

    Enlarge

  4. pREP3xN_ccdB

    Plasmid
    #79021
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79021-plasmid-map-sequence-id-151965

    Enlarge

  5. T4 Lysozyme T21C K124C WT*

    Plasmid
    #18243
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T21C K124C WT*
    Promoter
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  6. pREP82xN_ccdB

    Plasmid
    #79026
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79026-plasmid-map-sequence-id-147783

    Enlarge

  7. pREP81xN_ccdB

    Plasmid
    #79025
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79025-plasmid-map-sequence-id-151959

    Enlarge

  8. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

    Enlarge

  9. pREP41xN_ccdB

    Plasmid
    #79023
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79023-plasmid-map-sequence-id-151952

    Enlarge

  10. pREP42xN_ccdB

    Plasmid
    #79024
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Pernilla Bjerling, Göran Akusjärvi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79024-plasmid-map-sequence-id-147781

    Enlarge

  11. lentiCas9-Blast

    Plasmid
    #52962
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Lentiviral Prep
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52962-plasmid-map-sequence-id-322376

    Enlarge

  19. LentiGuide Cherry

    Plasmid
    #170510
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Pavel Tolar
    Article
    Newman et al biorXiv 2021.03.02.433398
    Inserts
    S. pyogenes sgRNA cassette
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170510-plasmid-map-sequence-id-343694

    Enlarge

Showing: 10141 - 10160 of 52614 results