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pDEST47-ARF4-GFP

Plasmid

#67392

Purpose

Mammalian expression of hArf4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
hnRNPA2_190-303

Plasmid

#118822

Purpose

Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

Depositor

Nicolas Fawzi

Article

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Use

Tags

His tag and TEV

Expression

Bacterial

Mutation

contains amino acids 190-302

Promoter

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3B G120A no-stop

Plasmid

#123206

Purpose

Gateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Glycine 120 to Alanine, stop codon removed

Promoter

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 WT-Puro

Plasmid

#123319

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET28a-FgßF3-ddFLN4-HIS-ybbr

Plasmid

#101743

Purpose

mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)

Use

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4, added 9F in FgßF3

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3−3xFLAG−AGO2(S387A)

Plasmid

#220462

Purpose

Express S387 phospho-null mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to alanine

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

last nucleotide of stop codon removed for c-termi…

Promoter

Availability

Academic Institutions and Nonprofits only
pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-HA

Plasmid

#162083

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-ProtC-HA

Use

Lentiviral

Tags

VSVg-ProtC-HA

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

Plasmid

#162084

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG

Use

Lentiviral

Tags

VSVg-ProtC-FLAG

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-AU1

Plasmid

#162085

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-ProtC-AU1

Use

Lentiviral

Tags

VSVg-ProtC-AU1

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG

Plasmid

#162086

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-HA-FLAG

Use

Lentiviral

Tags

VSVg-HA-FLAG

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-AU1

Plasmid

#162087

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-HA-AU1

Use

Lentiviral

Tags

VSVg-HA-AU1

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-VSVg-FLAG-AU1

Plasmid

#162088

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to VSVg-FLAG-AU1

Use

Lentiviral

Tags

VSVg-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

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pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-FLAG

Plasmid

#162095

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-HA-FLAG

Use

Lentiviral

Tags

ProtC-HA-FLAG

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpicVector3G_Puro-T2A-EGFP-ProtC-HA-AU1

Plasmid

#162096

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-HA-AU1

Use

Lentiviral

Tags

ProtC-HA-AU1

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1

Plasmid

#162097

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to ProtC-FLAG-AU1

Use

Lentiviral

Tags

ProtC-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpicVector3G_Puro-T2A-EGFP-HA-FLAG-AU1

Plasmid

#162098

Purpose

EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; EGFP linked to HA-FLAG-AU1

Use

Lentiviral

Tags

HA-FLAG-AU1

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-HA

Plasmid

#162103

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-ProtC-HA

Use

Lentiviral

Tags

VSVg-ProtC-HA

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

Plasmid

#162104

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG

Use

Lentiviral

Tags

VSVg-ProtC-FLAG

Expression

Mutation

WT

Promoter

EF1A

Availability

Academic Institutions and Nonprofits only

Enlarge