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Plasmid#219662PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (3 mutations) fused to mGreenLantern
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-PNPT1 S484A
Plasmid#223316PurposeLentiviral vector expressing human PNPT1 S484A variantDepositorInsertPolyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
UseLentiviralTagsExpressionMammalianMutationChanged serine 484 to alaninePromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Puro-PNPT1
Plasmid#223315PurposeLentiviral vector expressing human PNPT1DepositorInsertPolyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) K-Ras(WT)
Plasmid#224283PurposeCell transfection and expression of K-RasDepositorInsertK-Ras (KRAS Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVDnmt3AL_bGHpA
Plasmid#177347PurposeAAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsC-terminal domain of mouse Dnmt3l (194–415 aa), P…ExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVtet1_bGHpA
Plasmid#177351PurposeAAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterCMV promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-Antares
Plasmid#208852PurposeExpresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.DepositorInsertAntares
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-Nipah V
Plasmid#174228PurposeExpresses Nipah virus VDepositorInsertNipah V
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-RFX5
Plasmid#222545PurposePiggyBac transposon plasmid for doxycycline inducible expression of RFX5DepositorInsertRFX5 (RFX5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
LncRNA overexpression EF1α-BbsI-SV40polyA_puroR
Plasmid#219826PurposeOverexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 29, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits