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Plasmid#112151PurposeC. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)DepositorInsertC. thermophilum DASH/Dam1 complex
UseTagshis tag on Spc34 and strep tag on Dad1ExpressionMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERB130
Plasmid#58284Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1DepositorInsertsMis12-GFP-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA
UseTagsGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ66
Plasmid#24981DepositorInsertCbr-unc-119 Punc-122::GFP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDCB014
Plasmid#135433PurposeYeast integrating plasmid to express MCP-ABI and PYL1-VP64DepositorInsertsMCP-ABI
PYL1-VP64
UseTagsExpressionYeastMutationPromoterpAdh1 and pHHF2Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LZF1723: mI12b-EGFP
Plasmid#104123PurposesynOptopatch constructDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromotermI12bAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERB127
Plasmid#58283Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)DepositorInsertsMis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA
UseTagsGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
AByF
Plasmid#111084PurposeIntegrates marker gene onto D. melanogaster Y chromosomeDepositorInsertsY homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 bPAC cMyc T2A tDimer
Plasmid#85397Purposehumanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent proteinDepositorInsertshumanized photoactivated adenylyl cyclase
red fluorescent protein
UseAAVTagscMyc TagExpressionMammalianMutationPromoterhuman synapsin-1 and ribosomal skip sequence T2AAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-Cbx7-Delta-CD
Plasmid#82521PurposeLentivirus vector. Expresses mutant Halotag-Cbx7-Delta-CD fusion proteins in mammalian cells. Cbx7 chromodomain deletion deletion of amino acids 1–62.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationdeletion of amino acids 1–62PromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-H-RAN-H2A2B
Plasmid#106419PurposeH-RAN-H2A2B (RANbody H2A2B::Sm-HAtag)DepositorInsertH-RAN-H2A2B
UseTagsHA, His, and Multiple HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB4A5 JQ929582
Plasmid#38169DepositorInsertsmKate2
Superfolder GFP
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTLR-donor-UAS
Plasmid#119001PurposeRepair template for the TLR reporter with 10 UAS binding sites for yeast Gal4DepositorInsertmVenus
UseTagsExpressionMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTol1-uas-mAKT-2a-nlstdTomato
Plasmid#67706PurposeTol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomatoDepositorInsertmAKT-2A-nlstdTomato
UseTagsExpressionMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
BtuCD
Plasmid#92208PurposeExpression of BtuCD in E. coliDepositorUseTags10x Histidine Tag and Enterokinase Cleavage SiteExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXcX-G4BS-3.6TPH-EGFP
Plasmid#32572DepositorInsertEGFP
UseAdenoviralTagsExpressionMammalianMutationPromoter3.6TPHAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFGL815N
Plasmid#52323PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 68bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRAJ31f
Plasmid#39257DepositorInsertRAJ31 system (no terminator for the transRNA)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLlacO-1+pLtetO-1Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWY
Plasmid#112906PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWC
Plasmid#112903PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only