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Mm-PylRS-AF/Pyl-tRNACUA

Plasmid

#122650

Purpose

tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

Depositor

Howard Hang

Article

Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

Insert

PylRS-AF, Pyl-tRNACUA

Use

Tags

FLAG

Expression

Mammalian

Mutation

Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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VRK1_HUMAN_D0

Plasmid

#79684

Purpose

This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

Nicholas Levinson, Markus Seeliger, John Chodera

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK1 (VRK1 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
sgGFP_3

Plasmid

#78165

Purpose

sgGFP

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Use

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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CD9-mEGFP

Plasmid

#182864

Purpose

Localization: Tetraspanin/Membrane

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

human CD9 (CD9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Sema3g.a(L)-Fc-His

Plasmid

#72151

Purpose

Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3g.a (Sema3g Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-pDEST_TMEM192_BirA_Flag

Plasmid

#227958

Purpose

BioID experiment

Depositor

Alessandro Ori

Insert

TMEM192 (TMEM192 Human)

Use

Tags

BirA-FLAG

Expression

Mammalian

Mutation

R118G in BirA

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc-ns

Plasmid

#226719

Purpose

Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

pTEF1

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-NG-CBE C

Plasmid

#227422

Purpose

expresses the C-terminal of AAV-NG-CBE

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AAV-NG-CBE C-terminal

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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