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pMB1-spect-PthrC3_8-eGFP

Plasmid

#107411

Purpose

This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PthrC3_8

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBBR5pemIK-GW

Plasmid

#126556

Purpose

Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

Depositor

Drake Stenger, Lindsey Burbank

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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Salsa-BC2-P1A2

Plasmid

#202843

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet Response Element (TRE)

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b V12

Plasmid

#118323

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b V12 (RAP1B Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+5'MT-wbp2nl

Plasmid

#172467

Purpose

expresses myc-tagged version of wild-type protein

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl

Use

Xenopus expression

Tags

Myc

Expression

Mutation

Promoter

SP6

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b/GgVcl 1-1066 (full length)

Plasmid

#46171

Depositor

Susan Craig

Article

Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

Insert

Vcl (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Tags

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Salsa-BC6-P1A6

Plasmid

#202847

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet Response Element (TRE)

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC-eGFP

Plasmid

#107408

Purpose

This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PthrC

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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PEP119T

Plasmid

#98251

Purpose

Pseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

Gateway cassettee-HA-sfGFP11-11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b N17

Plasmid

#118322

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b N17 (RAP1B Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

S17N

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMJ839

Plasmid

#39317

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9 (NMA0631 Neisseria meningitidis)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pmGFP

Plasmid

#188451

Purpose

Expression of a modified GFP (mGFP) with short half-life

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Insert

Cyclin destruction box

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pU6_(Gluc)_INT(GenPurpClon)

Plasmid

#68433

Purpose

General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct

Use

CRISPR; Cloning vector for generating int-like co…

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 9

Plasmid

#36107

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 9

Use

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-neo-Strep_Flag_Nterm

Plasmid

#102644

Purpose

Epitope tag n-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al J Biol Chem. 2016 Sep 2;291(36):18818-42

Type

Empty backbone

Use

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-RanV19

Plasmid

#118316

Purpose

Expression of human Ran

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RanV19 (RAN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_9xD3B_Cluc_Norm

Plasmid

#68414

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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p123shy1-3HA

Plasmid

#67876

Purpose

encodes shy1 with a C-terminal 3xHA-tag under control of its endogenous promoter.

Depositor

Armin Djamei

Article

Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

Insert

shy1

Use

Expression in ustilago maydis

Tags

HA

Expression

Mutation

Promoter

shy1

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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