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Showing: 10081 - 10100 of 20487 results

  1. p426_Cas9_gRNA-ARS1206a

    Plasmid
    #87398
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87398-plasmid-map-sequence-id-193441

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  2. p426_Cas9_gRNA-ARS1414a

    Plasmid
    #87400
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1414a sequence GCGCCACAGTTTCAAGGGTC in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1414a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87400-plasmid-map-sequence-id-193443

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  3. p426_Cas9_gRNA-RDS1a

    Plasmid
    #87385
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87385-plasmid-map-sequence-id-193427

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  4. pETM6-1D4-mCherry

    Plasmid
    #73423
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1D4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1D4 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73423-plasmid-map-sequence-id-135323

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  5. pETM6-4A6-mCherry

    Plasmid
    #73424
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4A6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4A6 (orthogonal T7-lac variant)
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73424-plasmid-map-sequence-id-135324

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  6. pETM6-1E4-mCherry

    Plasmid
    #73426
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1E4 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1E4 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73426-plasmid-map-sequence-id-135326

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  7. pETM6-1B6-mCherry

    Plasmid
    #73420
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 1B6 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P1B6 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73420-plasmid-map-sequence-id-135320

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  8. pETM6-3H5-mCherry

    Plasmid
    #73419
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 3H5 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3H5 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73419-plasmid-map-sequence-id-135319

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  10. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid
    #92292
    Purpose
    Expression of humanized Mb2Cpf1
    Depositor
    Feng Zhang
    Insert
    huMb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92292-plasmid-map-sequence-id-181491

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  11. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid
    #92293
    Purpose
    Expression of humanized Mb3Cpf1
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92293-plasmid-map-sequence-id-181495

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  12. HCP8

    Plasmid
    #166110
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Whi5
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166110-plasmid-map-sequence-id-330382

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  13. pY113 (pcDNA3.1-huPb2Cpf1)

    Plasmid
    #92272
    Purpose
    Expression of humanized Pb2Cpf1
    Depositor
    Feng Zhang
    Insert
    huPb2Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92272-plasmid-map-sequence-id-181492

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  14. pY119 (pcDNA3.1-huLb5Cpf1)

    Plasmid
    #92295
    Purpose
    Expression of humanized Lb5Cpf1
    Depositor
    Feng Zhang
    Insert
    huLb5Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92295-plasmid-map-sequence-id-181498

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  15. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid
    #92267
    Purpose
    Expression of humanized TsCpf1
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    92267-plasmid-map-sequence-id-183046

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  16. pY118 (pcDNA3.1-huLb4Cpf1)

    Plasmid
    #92294
    Purpose
    Expression of humanized Lb4Cpf1
    Depositor
    Feng Zhang
    Insert
    huLb4Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92294-plasmid-map-sequence-id-181497

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  17. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  18. pY124 (pcDNA3.1-huBsCpf1)

    Plasmid
    #92300
    Purpose
    Expression of humanized BsCpf1
    Depositor
    Feng Zhang
    Insert
    huBsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92300-plasmid-map-sequence-id-181651

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  19. pDonorTp

    Plasmid
    #211788
    Purpose
    pDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpR
    Depositor
    Silvia Cardona
    Insert
    dhfr
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211788-plasmid-map-sequence-id-418339

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  20. pRPCE

    Plasmid
    #193712
    Purpose
    Expresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193712-plasmid-map-sequence-id-381728

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Showing: 10081 - 10100 of 20487 results