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pLCKO_AAVS1_sgRNA_2

Plasmid

#155085

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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ILK gRNA (BRDN0001147357)

Plasmid

#75909

Purpose

3rd generation lentiviral gRNA plasmid targeting human ILK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ILK (ILK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ILK gRNA (BRDN0001146314)

Plasmid

#75910

Purpose

3rd generation lentiviral gRNA plasmid targeting human ILK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ILK (ILK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ILK gRNA (BRDN0001144869)

Plasmid

#75911

Purpose

3rd generation lentiviral gRNA plasmid targeting human ILK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ILK (ILK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-R108K

Plasmid

#116292

Purpose

Lentiviral expression of EGFR R108K

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

R108K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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SIN-CMV-Cas9-V5-WPRE

Plasmid

#87904

Purpose

To produce lentiviral vector for gene editing

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

Cas9-V5

Use

Lentiviral

Tags

V5

Expression

Mutation

human codon-optimized

Promoter

CMV

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4#1/Cre

Plasmid

#173617

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLVU-coHPDL WT

Plasmid

#174128

Purpose

Expresses wild type codon-optimized HPDL in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Codon optimized

Promoter

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL WT 3xFlag-V5

Plasmid

#174129

Purpose

Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti GW-NL-myc

Plasmid

#113455

Purpose

Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

NanoLuc-cmyc

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG-RAB35 Q67L

Plasmid

#107105

Purpose

Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Article

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-UBE2G1 sg2R-P2A-Hygro

Plasmid

#124299

Purpose

Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

UBE2G1 (UBE2G1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

several silent mutations to disrupt sgRNA targeti…

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-VenusN

Plasmid

#27247

Depositor

Patrick Salmon

Insert

VenusNLS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti6-DJ1-V5-C106D

Plasmid

#29413

Depositor

Mark Cookson

Insert

DJ1 (PARK7 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Cys106Asp

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE-MAP2K4-G294E

Plasmid

#116430

Purpose

Lentiviral expression of MAP2K4 G294E

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K4 (MAP2K4 Human)

Use

Lentiviral

Tags

Expression

Mutation

G294E

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001145959)

Plasmid

#77832

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GNE gRNA (BRDN0001145858)

Plasmid

#77830

Purpose

3rd generation lentiviral gRNA plasmid targeting human GNE

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GNE (GNE Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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PNKP gRNA (BRDN0001147028)

Plasmid

#77162

Purpose

3rd generation lentiviral gRNA plasmid targeting human PNKP

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PNKP (PNKP Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-W-FUGW

Plasmid

#166751

Purpose

Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bcl-W (Bcl2l2 Mouse)

Use

Lentiviral

Tags

mCerulean3

Expression

Mutation

Promoter

human ubiquitin C

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-Puro

Plasmid

#110851

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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