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pAPM-D4 miR30-SUV39h2 ts1
Plasmid#115883PurposeSUV39h2 knockdownDepositorInsertSUV39h2 shRNA (SUV39H2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA-YFP-R5
Plasmid#106257PurposeExpresses Venus YFP fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertYellow fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of YFP af…ExpressionBacterialMutationPromoterpL-TetOAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21d_SNORD78_probe
Plasmid#73072Purposeantisense probe for detection of human SNORD78 (C/D box snoRNA U78)DepositorInsertSNORD78 (SNORD78 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS
Plasmid#107300PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS
Plasmid#107299PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAL4DBD-SuUR f.l
Plasmid#112995PurposeP element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genomeDepositorInsertSuUR (SuUR Fly)
UseP insertion vectorTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP
Plasmid#110828PurposeBacterial expression of E.coli DHFR N23PP mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgMTHFD1L_2
Plasmid#106317PurposeExpress Cas9 and sgRNA targeting MTHFD1LDepositorInsertsgRNA targeting MTHFD1L
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC45
Plasmid#104819PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-2). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hsp70:EB3-mKate2
Plasmid#105965Purposeheat shock inducible transgenesisDepositorInsertEB3 (MAPRE3 Human)
UseTagsmKate2ExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME GFP-DCX
Plasmid#105962Purposeentry clone for gateway cloningDepositorInsertNP_835365.1 (DCX Human)
UseTags6xHis-thrombin cleavage site and EGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o2
Plasmid#101310PurposeContains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.DepositorInsertoriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S137D (#628)
Plasmid#99944Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS137DPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMflT-o1
Plasmid#101309PurposeContains: oriC1 fragment of M. florum L1 replication origin (rpmH/dnaA intergenic region), colE1 replication origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.DepositorInsertoriC1 of M. florum strain L1 (rpmH/dnaA intergenic region)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 S103W (#623)
Plasmid#99941Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationS103WPromoterAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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