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pCLX-UBI-GFP

Plasmid

#27245

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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NbV5-eGFP

Plasmid

#201473

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-eGFP

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mT-Sapphire-N1

Plasmid

#54569

Purpose

Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May . 5(5):401-3.

Type

Empty backbone

Use

Tags

mT

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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SKIV2L

Plasmid

#156014

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SKIV2L (SKIC2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

D3E

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACt-mCherry-NES

Plasmid

#138215

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NES (Adcy10 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI825

Plasmid

#216633

Purpose

Cal-ID (V5) for AAV production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol (2024)

Insert

Cal-ID

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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ITIH4-bio-His

Plasmid

#52181

Purpose

Expresses full-length Inter-alpha-trypsin inhibitor heavy chain H4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITIH4 (ITIH4 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pUltra-MDH1-ME1

Plasmid

#184465

Purpose

Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydrogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Lentiviral

Tags

fused to T2A

Expression

Mammalian

Mutation

deletion of stop codon

Promoter

UbC

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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BRAC/pcDNA3

Plasmid

#51967

Purpose

mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

Depositor

Takeharu Nagai

Article

Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

Insert

BRAC

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pCD564

Plasmid

#153026

Purpose

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dxCas9(3.7), MCP-SoxS(R93A/S101A)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Promoter

Available since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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