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Plasmid#216068PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; BlastR; StopDepositorHas ServiceCloning Grade DNAInsertStop; IRES2_v1; Start; BlastR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA370
Plasmid#215988PurposeFragmid fragment: (N' terminus) attenuate expressionDepositorHas ServiceCloning Grade DNAInsertStart; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.6t
Plasmid#210258PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmScarlet
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CMV-SCLM
Plasmid#216758PurposeAAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleonDepositorInsertSuperClomeleon followed by WPRE and human growth hormone polyA terminator
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCMV (cytomegalovirus)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA337
Plasmid#214750PurposeMedium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validationDepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Wdr20-V5
Plasmid#217622PurposeMammalian expression of V5 tagged Drosophila Wdr20DepositorInsertWDR20
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFAST-Halotag-ER
Plasmid#214413PurposeER localization of CFAST fused to Halotag-ERDepositorInsertrat cb5 ERM
UseTagsCFAST-HaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-Halo-ER
Plasmid#214419PurposeER localization of low-affinity NFAST fused to Halo-ERDepositorInsertRspA-N
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-mito
Plasmid#214415PurposeMitochondria localization of low-affinity NFAST fused to mApple-mitoDepositorInsertRspA-N
UseTagsmApple-mitoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
NFAST(low)-mApple-6xHp
Plasmid#214412PurposeLipid droplet localization of low-affinity NFAST fused to mApple-6xHpDepositorInsertRspA-N
UseTagsmApple-6xHpExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.7t
Plasmid#210259PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertse2Crimson
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.3t
Plasmid#210255PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsDsRed
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421)
Plasmid#213619PurposeBacterial expression of SUMO-tagged rat syt1 (96-421)DepositorInsertsyt1 (96-421) (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 (96-421) I367C
Plasmid#213620PurposeBacterial expression of SUMO-tagged rat syt1 (96-421) I367CDepositorInsertsyt1 (96-421) I367C (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationC277A; I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403)
Plasmid#213621PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403)DepositorInsertsyt7 (136-403) (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…PromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2AB - 4A
Plasmid#213625PurposeBacterial expression of SUMO-tagged mouse syt7 C2AB - 4ADepositorInsertsyt7 C2AB - 4A (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, I298A, L361APromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 C2A-2A 2B
Plasmid#213626PurposeBacterial expression of SUMO-tagged mouse syt7 C2A-2A 2BDepositorInsertsyt7 C2A-2A 2B (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationF167A, F229A, R363HPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only