Addgene

We narrowed to 16,182 results for: multi

Showing: 10041 - 10060 of 16182 results

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pKI1.1

Plasmid

#85807

Purpose

CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Mutation

Promoter

AtRPS5A

Available since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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DS-NMcas

Plasmid

#48646

Purpose

Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
NM tracrRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

NM tracrRNA promoter and proC

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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AKARet-cyto

Plasmid

#96897

Purpose

FLIM sensor for PKA activation in cytosol

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for PKA Activity in Cytosol

Use

Tags

EGFP and sREAChet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAXY0017

Plasmid

#205586

Purpose

pNpuDnaE(C)_HygR(90-341)_3HA

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-HygR

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-rtTA

Plasmid

#32648

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
rtTA

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2011

Availability

Academic Institutions and Nonprofits only
pNSEN-d2

Plasmid

#59763

Purpose

Expresses nuclear EGFP and Tomato in mammalian cells

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

Inserts

d2-EGFP
Tomato

Use

Tags

3X NLS and PEST

Expression

Mammalian

Mutation

Promoter

CMV promoter and SV40 promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBPGUw-HACK-G4>QF2

Plasmid

#80277

Purpose

GAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB106-MTS-Citrine

Plasmid

#79135

Purpose

Binary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

SD3(1-50)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Rad18

Plasmid

#119003

Purpose

Mammalian expression of human Rad18 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Rad18

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-mCherry

Plasmid

#32643

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
mCherry

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
EKARet-nuc

Plasmid

#96896

Purpose

FLIM sensor for ERK activation in nucleus

Depositor

Ryohei Yasuda

Article

Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

Insert

FLIM Sensor for ERK Activity in Nucleus

Use

Tags

EGFP and sREAChet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-GFP1

Plasmid

#106418

Purpose

H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-GFP1

Use

Tags

HA, His, and Multiple HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDA183

Plasmid

#74247

Purpose

pSTL1-dPSTRr(2,1)

Depositor

Serge Pelet

Article

Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

Insert

pSTL1-dPSTRr(2,1)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
M-tdTom-SP

Plasmid

#48677

Purpose

Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Sp6

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
pH1-shControl-pSyn-tdtomato

Plasmid

#206319

Purpose

Control for pH1-shOPA1-pSyn-tdtomato

Depositor

Yusuke Hirabayashi

Article

Suga et al PLoS Biol. 2023 Aug 31;21(8):e3002246. d

Insert

shRNA scramble control

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929581

Plasmid

#38168

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only