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CDK5 gRNA (BRDN0001145725)

Plasmid

#77543

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5 (CDK5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgITGB1-1

Plasmid

#121533

Purpose

sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

Insert

sgITGB1-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PIK3CA gRNA (BRDN0001148028)

Plasmid

#77418

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CA (PIK3CA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_2

Plasmid

#102964

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_2 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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VCP F12.4 gRNA

Plasmid

#90939

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F12.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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VCP F11.4 gRNA

Plasmid

#90938

Purpose

3rd generation lentiviral gRNA plasmid targeting human VCP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

VCP (Guide Designation F11.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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CDK5 gRNA (BRDN0001148988)

Plasmid

#77544

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK5

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK5 (CDK5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM7 gRNA (BRDN0001147821)

Plasmid

#76112

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRPM7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRPM7 (TRPM7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164

Plasmid

#170169

Purpose

Lentiviral Expression of EF1a.SLC6A1.WT.EGFP

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

n/a

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1puro-shKIBRA-B

Plasmid

#40889

Depositor

Paul Reynolds

Article

Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.

Insert

shKIBRA-B (WWC1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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LRT2B-GO2

Plasmid

#136909

Purpose

Lentivirus expressing GO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO2

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

hU6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ESR1

Plasmid

#183288

Purpose

All-in-One CRISPRko system with a guide RNA that targets ESR1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ESR1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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LMTK3 gRNA (BRDN0001145703)

Plasmid

#77991

Purpose

3rd generation lentiviral gRNA plasmid targeting human LMTK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LMTK3 (LMTK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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LMTK3 gRNA (BRDN0001148227)

Plasmid

#77992

Purpose

3rd generation lentiviral gRNA plasmid targeting human LMTK3

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

LMTK3 (LMTK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-N-Ras(wildtype)-IRES-Puro

Plasmid

#120563

Purpose

Expresses HA-birA*-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mPRAS40

Plasmid

#15480

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (Akt1s1 Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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LCV2 V-1

Plasmid

#84804

Purpose

Cas9 with sgRNA targeting Exon 1 of VEGF-A

Depositor

Glenn Yiu

Article

Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

Insert

gRNA targeting VEGF-A (VEGFA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf2#1/Cre

Plasmid

#173643

Purpose

Expresses a Nf2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf2 (Nf2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-Flag-BRSK2_IDG-K

Plasmid

#135279

Purpose

Gateway destination clone of BRSK2 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

Depositor

Ben Major

Insert

Flag-BRSK2 (BRSK2 Human)

Use

Lentiviral; Gateway destination

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_DNMT1

Plasmid

#183282

Purpose

All-in-One CRISPRko system with a guide RNA that targets DNMT1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

DNMT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge