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Showing: 10001 - 10020 of 52000 results

  1. Nup133_mEGFP(-9b)

    Plasmid
    #163420
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163420-plasmid-map-sequence-id-321639

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  2. Nup133_mEGFP(-8b)

    Plasmid
    #163418
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163418-plasmid-map-sequence-id-321635

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  3. pZNF302-donor

    Plasmid
    #132513
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF302 (ZNF302 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132513-plasmid-map-sequence-id-275216

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  4. TP2_crtYB

    Plasmid
    #140233
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    na
    Promoter
    t7 promoter
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    140233-plasmid-map-sequence-id-288147

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  5. pTSHZ1-donor

    Plasmid
    #132479
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    132479-plasmid-map-sequence-id-274870

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  6. pENTR-R2L3-cmER-IRESpuro-pA

    Plasmid
    #139539
    Purpose
    Provides mouse ER protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    139539-plasmid-map-sequence-id-274701

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  7. pPATZ1-donor

    Plasmid
    #132522
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    PATZ1 (PATZ1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132522-plasmid-map-sequence-id-273934

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  8. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  9. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  10. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  11. C105m

    Plasmid
    #121008
    Purpose
    MoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-AAV ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    AAV degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121008-plasmid-map-sequence-id-236163

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  12. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  13. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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  14. C70m

    Plasmid
    #120982
    Purpose
    MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).
    Depositor
    Richard Murray
    Insert
    RpaR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120982-plasmid-map-sequence-id-236041

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  15. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  16. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120977-plasmid-map-sequence-id-236031

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  17. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120978-plasmid-map-sequence-id-236032

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  18. P61m

    Plasmid
    #120953
    Purpose
    MoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).
    Depositor
    Richard Murray
    Insert
    Tac promoter Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120953-plasmid-map-sequence-id-235967

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  19. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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  20. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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Showing: 10001 - 10020 of 52000 results