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Plasmid#57696PurposeLocalization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertZyxin (ZYX Human)
UseTagstdEosExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mKO-Vinculin-23
Plasmid#57852PurposeLocalization: Focal Adhesions, Excitation: 548, Emission: 561DepositorInsertVinculin (VCL Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Delta-ATG1-mMCL1
Plasmid#45823PurposeThis plasmid was made to show that the two MCL-1 bands DO NOT arise from a non-canonical translation initiation downstream of the start codon.DepositorInsertDelta ATG1-mMCL1
UseTagsExpressionMammalianMutationThe first amino acid (ATG) is deleted from the fu…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
DCT.VV.Orf200
Plasmid#12888PurposeThis plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.DepositorInsertVV.Orf200
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 29, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F3.loxP
Plasmid#63200PurposepCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.K6.loxP
Plasmid#63203PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAV10.HO5.loxP
Plasmid#63206PurposepCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Stag-ComMT
Plasmid#215159PurposeEncodes a proteobacteria metallothionein fused with the S-tag at the N-terminusDepositorInsertStag-ComMT
UseTagsS-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Alpha
Plasmid#177961PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Alpha: del69,70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H)DepositorInsertSARS-CoV-2 Spike protein (Alpha: del69, 70, del144, D614G, N501Y, A570D, P681H, T716I, S982A, D1118H) (S )
UseTagsExpressionMammalianMutationdel69, 70, del144, D614G, N501Y, A570D, P681H, T7…PromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G-Gamma
Plasmid#177963PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)DepositorInsertSARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )
UseTagsExpressionMammalianMutationL18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…PromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG1160 mCherry-FIREtag
Plasmid#205164PurposeExpresses mCherry -FIREtag in mammalian cellsDepositorInsertmCherry-FIREtag
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
DS-SPcas
Plasmid#48645PurposeBacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycinDepositorInsertsCas9
tracrRNA precursor
UseCRISPRTagsExpressionMutationPromoterproC and tracrRNA promoterAvailable sinceDec. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) EC Her2-Fc
Plasmid#183250PurposeSoluble expression of the extracellular domain of the HER2 antigen. Human IgG1 Fc.DepositorInsertHer2 antigen Fc
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG1344 EGFP-FIREtag
Plasmid#205165PurposeExpresses EGFP-FIREtag in mammalian cellsDepositorInsertEGFP-FIREtag
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBF3060
Plasmid#26853DepositorInsertCreA recombinase
UseCre/LoxTagsExpressionYeastMutationN/APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBF3038
Plasmid#26850DepositorInsertCreA recombinase
UseCre/LoxTagsExpressionYeastMutationN/APromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4137384
Plasmid#149543PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike) for VSV pseudotypingDepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationH. sapiens recodePromoterCMVAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
M-ST1cas
Plasmid#48669PurposeMammalian S. thermophilus #1 Cas9 expression, human optimizedDepositorInsertCas9
UseCRISPRTagsNLSExpressionMutationPromoterCMVAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only