Addgene

Showing: 9981 - 10000 of 24295 results

Results per page:

Sort results by:

Pt NaV1.4

Plasmid

#200233

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Dt NaV1.4

Plasmid

#200250

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Dendrobates tinctorius NaV1.4 – muscle channel

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM069

Plasmid

#216821

Purpose

Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

Depositor

Ken Sawin

Article

Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

Insert

mStayGold2

Use

Tags

RIPGLIN and VinnyLinker

Expression

Bacterial

Mutation

Aspergillus nidulans codon-adjusted

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-Lumiscarlet

Plasmid

#208854

Purpose

Expresses FLAG epitope-tagged Lumiscarlet "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

Lumiscarlet

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-dKeNLuc

Plasmid

#208856

Purpose

Expresses FLAG epitope-tagged dKeNLuc "Red" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

dKeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-CeNLuc

Plasmid

#208838

Purpose

Expresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-CeNL

Plasmid

#208839

Purpose

Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-GeNL

Plasmid

#208841

Purpose

Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-YeNL

Plasmid

#208849

Purpose

Expresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

YeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMV:Flag-OeNL

Plasmid

#208850

Purpose

Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Use

Tags

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-HGM (optimized)

Plasmid

#220988

Purpose

Adeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion construct

Depositor

Joshua Kritzer

Article

Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

Insert

HaloTag-GFP-mito

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2_-87prox1a:basEGFP_Acry:GFP

Plasmid

#218213

Purpose

Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-87prox1a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TR-Csy4-R115A/R119A

Plasmid

#80696

Purpose

Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4-R115A/R119A

Use

AAV

Tags

Expression

Mammalian

Mutation

Arg115 and Arg119 both mutated to Ala

Promoter

CBA

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-601[insert(+1) TA-rich]

Plasmid

#221196

Purpose

601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

601[insert(+1)TA-rich]

Use

Unspecified

Tags

Expression

Mutation

601 has an additional mutation added 22 bp from d…

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-ScChd1[R126A/R130A]

Plasmid

#221197

Purpose

ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

R126A, R130A

Promoter

T7

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Use

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Promoter

Available since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL7086

Plasmid

#167250

Purpose

orthogonal Mango reporter output for cell-free expression

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-Mango

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
95.a_pSTC0-zeo

Plasmid

#202334

Purpose

Design 95.a in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

95.a

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
95.b_pSTC0-zeo

Plasmid

#202335

Purpose

Design 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

95.b

Use

Tags

Expression

Mutation

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge