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Plasmid#86756PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationS29APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-DNMT3a ts1
Plasmid#115865PurposeDNMT3a knockdownDepositorInsertDNMT3a shRNA (DNMT3A Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p20StAD
Plasmid#190268PurposeConstitutively expresses a protein of interest fused to a GAL4 transcription activating domain. Targeted integration to genomic locus YPRC-delta15, Chromosome XVI.DepositorTypeEmpty backboneUseSynthetic Biology; Yeast genome-integrating vectorTagsGAL4-ADExpressionMutationPromoterTDH3Available sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#84257PurposeExpresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mKate2
Plasmid#162624PurposeAllows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assaysDepositorInsertmKate2
UseTagsExpressionMammalianMutationPromoterSP6Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd-Track Flag GCN5 Y621A/P622A
Plasmid#14425DepositorInsertGCN5 Y621A/P622A (KAT2A Human)
UseAdenoviralTagsflagExpressionMammalianMutationY621A, P622A. Catalytically inactive acetyltransf…PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF75-2xCSTREP
Plasmid#136234PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF75DepositorInsertORF75
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-DNMT3a ts2
Plasmid#115866PurposeDNMT3a knockdownDepositorInsertDNMT3a shRNA (DNMT3A Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mGnptg
Plasmid#78106PurposeMammalian expression of mouse Gnptg with C-terminal His tagDepositorInsertGnptg (Gnptg Mouse)
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AKAR
Plasmid#181844PurposeGenetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCL9-BirA*
Plasmid#109147PurposeTo generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertBCL9 (BCL9 Human)
UseTagsBirA(R118G)ExpressionMammalianMutationPromoterCMV/TetO2Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13
Plasmid#157713Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13 (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
UseTagsExpressionBacterialMutationStop codon to glutamine at 23rd amino acidPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_ARHGAP4-1/1
Plasmid#91518PurposeProtein expression and purification of human SH3 domain construct ARHGAP4-1/1DepositorInsertARHGAP4-1/1 (ARHGAP4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP9-1/1
Plasmid#91442PurposeProtein expression and purification of human SH3 domain construct ARHGAP9-1/1DepositorInsertARHGAP9-1/1 (ARHGAP9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO
Plasmid#205463PurposeA backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-A; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO
Plasmid#205465PurposeA backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmidDepositorInsertMTS; N136; ccdb; RsDddA-C; UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF8-2xCSTREP
Plasmid#136172PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF8DepositorInsertORF8
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-K8b-2xCSTREP
Plasmid#136159PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8bDepositorInsertK8b
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only