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Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
UseTagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX-hN1ICD
Plasmid#91897PurposeDoxycycline inducible expression of human Notch1 ICDDepositorInsertNotch1 intracellular domain (NOTCH1 Human)
UseLentiviralTagsExpressionMammalianMutationcodons 1770 to 2555 of human NOTCH1PromoterTRE promoter, Tet ONAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
CLIMP-63 pcDNA
Plasmid#80977Purposemammalian expression of CLIMP-63DepositorInsertCLIMP-63 (CKAP4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-U6
Plasmid#120358PurposepiggyBac transposon vector for sgRNA expression.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16
Plasmid#172746PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-16
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s …ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S i…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP670nano3
Plasmid#184666PurposeHuman alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3DepositorInsertmiRFP670nano3-human alpha-tubulin
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only