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Plasmid#164918PurposeCEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNADepositorInsertP(Z)-mcherry
UseCRISPRTagsExpressionYeastMutationPromoterP(Z), synthetic promoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM254 - sgRNA mosTI neoR
Plasmid#159831PurposeSingle copy or array insertion by MosTI using split NeoR selectionDepositorInsertsgRNA mosTI neoR
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC1)_Psyn-sgRNArodA
Plasmid#149659Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC1), PsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10AC2)_Psyn-sgRNArodA
Plasmid#149660Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10AC2), PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAmreB
Plasmid#149595Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAftsI
Plasmid#149591Purposeall-in-all CRISPRi vector for targeting B. burgdorferi ftsIDepositorInsertdCas9, lacI, sgRNAftsI
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNArodA
Plasmid#149657Purposeall-in-one CRISPRi vector for targeting B. burgdorferi rodADepositorInsertdCas9, lacI, sgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(-10TC)_Psyn-sgRNAflaB
Plasmid#149589Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseTagsExpressionBacterialMutationPromoterPflaB, PpQE30(-10TC), PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Ddx42-L
Plasmid#122343PurposeExpresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ddx42
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Ddx42-R
Plasmid#122344PurposeExpresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ddx42
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Ncapd3-R
Plasmid#122330PurposeExpresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ncapd3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Npm1-R
Plasmid#122331PurposeExpresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Npm1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Npm1-L
Plasmid#122332PurposeExpresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Npm1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dhx15-R
Plasmid#122335PurposeExpresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Dhx15
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Dhx15-L
Plasmid#122336PurposeExpresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Ddx15
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Birc6-L
Plasmid#122337PurposeExpresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Birc6
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Birc6-R
Plasmid#122338PurposeExpresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Birc6
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Trim28-R
Plasmid#122339PurposeExpresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Trim28
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Trim28-L
Plasmid#122340PurposeExpresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Trim28
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-Trim27-L
Plasmid#122317PurposeExpresses sgRNA targeting mouse Trim27 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Trim27
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only