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pET28b-MseLig1

Plasmid

#107071

Purpose

Expresses M. sedula DNA Ligase 1 in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

DNA ligase 1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b-dabAB2

Plasmid

#133013

Purpose

pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

6xHis, StrepII tag, and sfGFP

Expression

Bacterial

Mutation

Promoter

t7

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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A104T DJ-1 pET15b

Plasmid

#60680

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

A104T DJ-1 pET15b (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

A104T

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET-BoNT/X

Plasmid

#168017

Purpose

Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

BoNT/X-LC(1-439)-6xHis

Use

Tags

Hisx6

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET23(a)_mod

Plasmid

#26363

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FLAG/HA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Article

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pET21b-RL016A

Plasmid

#42135

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mYPet-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET-UbcH10-DN

Plasmid

#8502

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Use

Tags

His

Expression

Bacterial

Mutation

Dominant Negative, C114S

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
PET11a-Af2sir2

Plasmid

#8383

Depositor

Cynthia Wolberger

Article

Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Insert

Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)

Use

Tags

Expression

Bacterial

Mutation

PET11a-Af2Sir2D103N

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pET20b-pIgR D1-H6

Plasmid

#12530

Depositor

Pamela Bjorkman

Article

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Use

Tags

His

Expression

Bacterial

Mutation

Lys 1-Val 109

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pET28-GG-PUF

Plasmid

#52137

Purpose

Receiving vector for PUM-HD assembly and expression in E.coli

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Use

Tags

PUM-HD flanking region 1' and PUM-HD flankin…

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET30b GluBP600n

Plasmid

#119835

Purpose

Production of recombinant GluBP600n high affinity protein in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

GluBP600n

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET32b-FLJ23445

Plasmid

#15434

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

FLJ23445

Use

Tags

His and Trx

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET28a(+)/FaGH16A

Plasmid

#100910

Purpose

Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH16A

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-EgC*

Plasmid

#20378

Depositor

Andrew Lee

Article

Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

Insert

Eglin C wt*

Use

Tags

Expression

Bacterial

Mutation

F10W

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only

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pET14b-mEKLF

Plasmid

#67834

Purpose

Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

Depositor

James Bieker

Article

Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

Insert

EKLF (Klf1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

T7

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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PL-452 C-CyPet

Plasmid

#19176

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

Insert

CyPet

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only