-
Plasmid#222220PurposeFor recombinant expression of vanA (PP_3736 from Pseudomonas putida) with a C-terminal His tagDepositorInsertvanA putida (PP_RS19450 Pseudomonas putida KT2440)
UseTags6x HisExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB111
Plasmid#222210PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tagDepositorInsertfghA(G76V)
UseTags6x HisExpressionBacterialMutationChanged glycine 76 to valinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB112
Plasmid#222211PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tagDepositorInsertfghA(S11R)
UseTags6x HisExpressionBacterialMutationChanged Serine 11 to ArgininePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACB113
Plasmid#222212PurposeFor recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tagDepositorInsertfghA(W15C)
UseTags6x HisExpressionBacterialMutationChanged Tryptophan 15 to CysteinePromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dual-CRISPR-U6-H1(pBP43)
Plasmid#218930PurposeThe backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6 and H1Available sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-dual-scafffold-2.0 (pBP49)
Plasmid#218931PurposeThe dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.DepositorInsertdual scaffolds for CRISPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A_R
Plasmid#200183PurposeWT PA14 I-F Leader and G1A-T2A RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
UseTagsExpressionBacterialMutationG1A-T2A in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A-A28C_R
Plasmid#200185PurposeWT PA14 I-F Leader and G1A-T2A-A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
UseTagsExpressionBacterialMutationG1A-T2A-A28C in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-457
Plasmid#200213PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 H25A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 H25A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-458
Plasmid#200214PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 E184A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 E184A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-459
Plasmid#200215PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas1 K214A)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas1 K214A mutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-460
Plasmid#200216PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-461
Plasmid#200217PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAW-462
Plasmid#200218PurposeStrep-TEV-PaCas1 + PaCas2/3 (Cas2 K11D, R12E, R55E, N56D)DepositorInsertPaCas1, PaCas2-3
UseTagsStrep II-TEV on Cas1ExpressionBacterialMutationCas2 K11D-R12E, R55E-N56D mutationsPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
UseTagsExpressionBacterialMutationPromoterpXYLAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES
Plasmid#182482PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free PcPTS
Plasmid#182483PurposeYeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS
PTS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (AG4TGGA)2
Plasmid#182488PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free (PT)4P
Plasmid#182490PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
noSIIN
Plasmid#185666PurposeshApc-expressing lentiviruses with no expression of SIINFEKLDepositorInsertsmScarlet
miR-30a
shApc
UseLentiviralTagsExpressionMutationPromoterEf1aAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only