Addgene

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pGC630

Plasmid

#107013

Purpose

GFP reporter of lag-2 driven by 2 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

Expression

Worm

Mutation

Promoter

lag-2

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFM110

Plasmid

#40156

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SE (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

7SE; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM107

Plasmid

#40153

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, 7SA (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

7SA; base pairs 6-523 of par-2 were recoded to c…

Promoter

tac

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pFM103

Plasmid

#40149

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, wild-type (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

tac

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM109

Plasmid

#40155

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, C56S (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM108

Plasmid

#40154

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, S241A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM106

Plasmid

#40152

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R163A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM105

Plasmid

#40151

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, K162A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

tac

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM104

Plasmid

#40150

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant, R183-5A (par-2 Nematode)

Use

Tags

GST

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded …

Promoter

T7

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1 AT1AR-TSTS/4A

Plasmid

#45634

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-TSTS/A (Agtr1a Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Thr332, Ser335, Thr336, and Ser338 all changed to…

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pFM034

Plasmid

#40137

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (wild-type) (par-2 )

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

pie-1

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Bcl2

Plasmid

#17999

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-Bcl2

Plasmid

#18003

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP-C2-hSphK2

Plasmid

#84370

Purpose

Expression of AcGFP-SphK2 fusion protein in mammalian cells

Depositor

Anthony Don, Hugh Rosen

Article

Don et al Biochem Biophys Res Commun. 2009 Feb 27;

Insert

SPHK2 (SPHK2 Human)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only
pCAG.Myc-SARS2_3CL

Plasmid

#200120

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pIFU002

Plasmid

#174299

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HA and cMyc

Expression

Yeast

Mutation

N343Q, N501Y

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIFU001

Plasmid

#174298

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

HA and cMyc

Expression

Yeast

Mutation

N343Q, E484K

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEnt L1L3 EF1a-tTA-3

Plasmid

#24415

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2 GW delCMV

Plasmid

#29496

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge