Addgene

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pMZ039

Plasmid

#67757

Purpose

Expresses N-terminal kinase domain of Chk1 with C-term GFP fluorescent tag

Depositor

Michael Lampson

Article

Zhang et al Cell Cycle. 2014;13(24):3828-38. doi: 10

Insert

zebrafish Chk1

Use

Tags

GFP

Expression

Mutation

only encodes N-term kinase domain (amino acids 1-…

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_wt-IRES-GFP

Plasmid

#26491

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_2A-IRES-GFP

Plasmid

#26545

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

SD1 G->A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPA-IRES-GFP

Plasmid

#26547

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7_LPA

Use

Retroviral

Tags

Expression

Mammalian

Mutation

loop mutant A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPB-IRES-GFP

Plasmid

#26548

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7_LPB

Use

Retroviral

Tags

Expression

Mammalian

Mutation

loop mutant B

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPC-IRES-GFP

Plasmid

#26549

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7_LPC

Use

Retroviral

Tags

Expression

Mammalian

Mutation

loop mutant C

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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MXW-PGK-let-7_LPD-IRES-GFP

Plasmid

#26551

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Aug 31. ():.

Insert

cel-pre-let-7_LPD

Use

Retroviral

Tags

Expression

Mammalian

Mutation

loop mutant D

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax WT

Plasmid

#23284

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-2

Plasmid

#26807

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

L7V, G76S, K395R and I482L

Promoter

CMV

Available since

July 26, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST17-TARG1

Plasmid

#172594

Purpose

For purification (from E.coli) of His-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1

Plasmid

#47888

Purpose

Expresses myc tagged Miro1

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 (RHOT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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hIRE1a wt

Plasmid

#20744

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1

Plasmid

#18881

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2

Plasmid

#47891

Purpose

Expresses myc tagged Miro2

Depositor

Pontus Aspenström

Article

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro2 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Tags

Expression

Mutation

Promoter

L-arabinose-inducible promoter PBAD

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TfR-OVA

Plasmid

#64600

Purpose

Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

Depositor

Martin Zenke, Sandra Diebold

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Use

Tags

Transmembrane domain of Transferrin Receptor (TfR…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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p15A_SpyCas9_CmR

Plasmid

#119162

Purpose

Expresses S. pyogenes Cas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

Cas9 from S. pyogenes (NEWENTRY )

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRATIO4212

Plasmid

#141313

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPIGpluc

Plasmid

#35645

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

CB

Available since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

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