Addgene

We narrowed to 7,571 results for: Dos

Showing: 1961 - 2000 of 7571 results

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p-sfGFP-pA-FRT-Amp-FRT

Plasmid

#176620

Purpose

for generating PCR templates with sfGFP and Amp for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

super folder green fluorescent protein with FRT-flanked ampicillin cassette

Use

Unspecified

Tags

Expression

Mutation

H217R - please see dep. comments

Promoter

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pM80

Plasmid

#173173

Purpose

HA-dCas9

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pSWD95Y14D

Plasmid

#159098

Purpose

CckA FRET Sensor with cyclic-di-GMP point mutant

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3

Use

Tags

Expression

Bacterial

Mutation

Mutated Y514 to D514

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2042

Plasmid

#139927

Purpose

Complementation plasmid for LetAB with 4 ring (rings 1347) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB (1-159; 280-515; 747-877)

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2045

Plasmid

#139924

Purpose

Expression plasmid for 4 ring (rings 1347) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB (43-159; 280-515; 747-877)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2002

Plasmid

#139887

Purpose

Expression plasmid for LetAB with 4 ring (rings 1567) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta161-514)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL2001

Plasmid

#139886

Purpose

Expression plasmid for LetAB with 3 ring (rings 167) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta161-634)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-VASA-tdTomato-pNeo

Plasmid

#141030

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-U6-BsmBI-R5

Plasmid

#141032

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-cjSTELLA-mCerulean-PuroTK

Plasmid

#141029

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

mCerulean

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-P3P1r

Plasmid

#141014

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R2-pA-L4

Plasmid

#141062

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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CSIV-DEST

Plasmid

#141053

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R4

Plasmid

#141054

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R5R4

Plasmid

#141055

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R4-H1-BsmBI-L2

Plasmid

#141034

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R4-H1-BbsI-L2

Plasmid

#141033

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L1-U6-BbsI-R5

Plasmid

#141031

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-R2L4

Plasmid

#141061

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HxHLICNot

Plasmid

#140183

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

12xHis

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0055

Plasmid

#117622

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1859

Plasmid

#123065

Purpose

Positive regulator of Ste20

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste50 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS511

Plasmid

#123063

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS510

Plasmid

#123062

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig1 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS509

Plasmid

#123061

Purpose

Negative regulator of Fus3

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Msg5 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS081

Plasmid

#123060

Purpose

Regulator of G protein signalling

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Sst2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS930

Plasmid

#123054

Purpose

Beta-estradiol inducible DNA binding domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Z3E [3a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS626

Plasmid

#123045

Purpose

P-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Mam2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS495

Plasmid

#123043

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pRNR2m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS497

Plasmid

#123042

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pCUP1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS496

Plasmid

#123041

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pCYC1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS498

Plasmid

#123040

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pPHO5m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS486

Plasmid

#123039

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pGAL1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS493

Plasmid

#123037

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pALD6m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS487

Plasmid

#123036

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTDH3m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS491

Plasmid

#123035

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTEF2m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS494

Plasmid

#123034

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pRNR1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS485

Plasmid

#123033

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (1x) [2a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1961 - 2000 of 7571 results

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