Skip to main content
Addgene
Showing: 81 - 100 of 217 results

  2. SiC-V2-Cas9G7

    Plasmid
    #133043
    Purpose
    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.
    Depositor
    Sriram Neelamegham
    Article
    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1
    Insert
    Tet repressor
    Use
    Lentiviral
    Tags
    Cerulean
    Expression
    Mutation
    Promoter
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    133043-plasmid-map-sequence-id-264424

    Enlarge

  5. pTRE2-Hyg-EGFP

    Plasmid
    #177152
    Purpose
    EGFP for the tet-off system
    Depositor
    Bertrand Collet
    Insert
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177152-plasmid-map-sequence-id-353296

    Enlarge

  9. pAGN33

    Plasmid
    #131105
    Purpose
    An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants
    Depositor
    Riccardo Manganelli
    Article
    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1
    Insert
    P-furA102tetO-pip
    Use
    Tags
    Expression
    Bacterial
    Mutation
    the furA102 promoter was mutated with the inserti…
    Promoter
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131105-plasmid-map-sequence-id-262707

    Enlarge

  17. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Use
    Tags
    3x-FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    pTet
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

    Enlarge

Showing: 81 - 100 of 217 results