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We narrowed to 402 results for: ta cloning vector

Showing: 81 - 100 of 402 results

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pFRT-TODestFLAGHA_RBPMS_R38Q

Plasmid

#65094

Purpose

Destination vector with RBPMS with R38Q mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to glutamine

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_R38A_E39A

Plasmid

#65664

Purpose

FLAG-HA expression vector with RBPMS carrying mutation R38A, E39A

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed arginine 38 to alanine, and glutamic acid…

Promoter

CMV, 2x Tet

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-FL_WobbleMut

Plasmid

#209053

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Insert

TAF5 full length

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-FL

Plasmid

#209047

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Insert

TAF5 full length

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8

Plasmid

#209048

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-Usp9x_CD-mCherry

Plasmid

#170380

Purpose

Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

Depositor

Miguel Ramalho-Santos

Article

Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

Insert

Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)

Use

Tags

mCherry-3xFlag

Expression

Mammalian

Mutation

Catalytic domain

Promoter

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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Zebrabow-GateDest

Plasmid

#118221

Purpose

For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

Depositor

Alex Schier, Albert Pan

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L - ccdB survival cassette

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS2_HindIII

Plasmid

#65666

Purpose

FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pYSD_lambda_mRFP

Plasmid

#217831

Purpose

Yeast surface display Fab vector with lambda light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD_kappa_mRFP

Plasmid

#217830

Purpose

Yeast surface display Fab vector with kappa light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_lambda

Plasmid

#217829

Purpose

Mini-mutagenesis plasmid for lambda light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_kappa

Plasmid

#217828

Purpose

Mini-mutagenesis plasmid for kappa light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR

Plasmid

#65091

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed sequence to render plasmid resistant to s…

Promoter

CMV, 2x Tet

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestMYC_RBPMS_KDR

Plasmid

#65667

Purpose

MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

MYC

Expression

Mammalian

Mutation

introduced HindIII site to exchange FLAG-HA tag w…

Promoter

CMV, 2x Tet

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS_KDR_K100E

Plasmid

#65093

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 100 to glutamic acid (KDR backgrou…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR_F65A

Plasmid

#65092

Purpose

Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

change phenylalanine 65 to alanine (KDR backbone)

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_K36E_R38E

Plasmid

#65095

Purpose

Destination vector with RBPMS with K36E/R38E mutation for stable cell line generation

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

change lysine 36 to glutamic acid and arginine 38…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_KDR_K100A

Plasmid

#65663

Purpose

FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 100 to alanine (KDR background)

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A

Plasmid

#65658

Purpose

FLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, E39A

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

changed lysine 36 to glutamic acid, arginine 38 t…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 81 - 100 of 402 results

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