Showing: 81 - 100 of 267 results
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Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pCL52.2
Plasmid#13656DepositorTypeEmpty backboneUseE. coli-s. arueus shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSR1002
Plasmid#208735PurposeEmpty backbone containing the S. aureus LAC-p01 repliconDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA-G83I
Plasmid#191239PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and Glycin…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-CP8B1
Plasmid#191240PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A32
Plasmid#191241PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-1A31
Plasmid#191242PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
UseTagsnuclease A from S. aureus fused to the poly-Leu T…ExpressionBacterialMutationChanged Alanine 112 to Cysteine in SN, and predic…PromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pYL112-19
Plasmid#15459DepositorInsertS. aureus phage L54a integrase
UseS.aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
GST-MNase
Plasmid#136291PurposeExpresses Micrococcal nucleaseDepositorInsertMNase
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKOR1
Plasmid#133446PurposeEscherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selectionDepositorTypeEmpty backboneUseE.coli/s.aureus shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNM58
Plasmid#128409PurposeSystem with AIP and xylose inducible agrCA sensorDepositorInsertsargCA
gfpmut2
UseTagsExpressionBacterialMutationPromoterP3 and PxylRAvailabilityAcademic Institutions and Nonprofits only -
pET15TV-L-SaODH
Plasmid#110804PurposeExpresses SaODH and opine dehydrogenase from Staphylococcus aureusDepositorInsertSAV2468 (SAV2468 )
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSR1048
Plasmid#208738PurposeCharacterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)DepositorInsertPRelA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPRelAAvailabilityAcademic Institutions and Nonprofits only -
pSR1051
Plasmid#208739PurposeCharacterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)DepositorInsertPZwf
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPZwfAvailabilityAcademic Institutions and Nonprofits only -
pSR1054
Plasmid#208740PurposeCharacterization plasmid for PAmy (sfgfp expressed from PAmy on the S. aureus pC194 replicon)DepositorInsertPAmy
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPAmyAvailabilityAcademic Institutions and Nonprofits only -
pSR1055
Plasmid#208741PurposeCharacterization plasmid for PHrcA (sfgfp expressed from PHrcA on the S. aureus pC194 replicon)DepositorInsertPHrcA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPHrcAAvailabilityAcademic Institutions and Nonprofits only -
pSR1057
Plasmid#208742PurposeCharacterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)DepositorInsertPPgk
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPPgkAvailabilityAcademic Institutions and Nonprofits only -
pSR1058
Plasmid#208743PurposeCharacterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)DepositorInsertPHbs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPHbsAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 267 results