Addgene

We narrowed to 1,125 results for: smo

Showing: 81 - 100 of 1125 results

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pLSSmOrange-alpha-Tubulin

Plasmid

#37137

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

human alpha-tubulin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-YFP-Smo

Plasmid

#31618

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

YFP

Expression

Mammalian and Worm

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-SNAP-Smo

Plasmid

#31617

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

SNAP

Expression

Mammalian and Worm

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGEX mSmo-Cterm

Plasmid

#58702

Purpose

used for anti-mouse Smoothened antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

tac

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
SmoM2 (W535L)-pcw107

Plasmid

#64627

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

SMO (SMO Human)

Use

Lentiviral

Tags

Expression

Mutation

W535L

Promoter

PGK

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-SNAP-Smo

Plasmid

#31619

Depositor

Rajat Rohatgi

Article

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Insert

Smo (Smo Mouse)

Use

Retroviral

Tags

SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGE-mSmo-YFP

Plasmid

#38094

Depositor

Philip Beachy

Article

Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

Insert

Smo (Smo Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-mSmo-NQ4-V5

Plasmid

#126416

Purpose

Localization, Expression of Smoothened NQ4 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Smo glycosylation site NQ4

Promoter

Available since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET28 mSmo-Cterm

Plasmid

#58703

Purpose

used for anti-mouse Smoothened antibody production (for affinity purification)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Smo (Smo Mouse)

Use

Tags

6X-HIS

Expression

Bacterial

Mutation

contains C terminus (aa 543-793)

Promoter

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-SMO-1(GG)

Plasmid

#87054

Purpose

Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

smo-1 (smo-1 Nematode)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The last aa was removed to render processed SMO-1…

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II-YPet(short)

Plasmid

#118714

Purpose

The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

Desmoplakin II-YPet(short) (DSP Human)

Use

Retroviral

Tags

YPet(short)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-lssmOrange

Plasmid

#179795

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

lss mOrange

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmooth_AA

Plasmid

#148228

Purpose

Insect Expression of DmSmooth

Depositor

Elisa Izaurralde

Insert

DmSmooth (sm Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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MOSMO_pcDNA6.2/EmGFP-Bsd

Plasmid

#176975

Purpose

Mammalian expression vector encoding MOSMO and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MOSMO (C16orf52 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ4

Plasmid

#126407

Purpose

Expression of Smoothened NQ4 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Smo glycosylation site NQ4

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ3

Plasmid

#126406

Purpose

Expression of Smoothened NQ3 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Smo glycosylation site NQ3

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-MosMos

Plasmid

#120515

Purpose

ssRNA expression

Depositor

Petr Svoboda

Insert

Mos (Mos Mouse)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange-DEVD-mKate2

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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1105-Desmoglein2-FLAG-eGFP

Plasmid

#36989

Depositor

Kathleen Green

Article

Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

Insert

Dsg2 (DSG2 Human)

Use

Tags

FLAG and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPSmOrange-Kan

Plasmid

#44951

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPSmOrange

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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