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Showing: 81 - 100 of 49183 results

  4. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-403308

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  6. BcLOV4 fusion tools screening plasmid #1

    Plasmid
    #174511
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Article
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Insert
    [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174511-plasmid-map-sequence-id-357205

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  7. BcLOV4 fusion tools screening plasmid #2

    Plasmid
    #174512
    Purpose
    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Article
    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52
    Insert
    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    Use
    Tags
    3xFLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    174512-plasmid-map-sequence-id-357202

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  10. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  12. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid
    #169733
    Purpose
    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169733-plasmid-map-sequence-id-334140

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  14. ppk301-T2A-QF2 HDR plasmid

    Plasmid
    #130667
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene
    Depositor
    Leslie Vosshall
    Article
    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1
    Insert
    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    Use
    Donor template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130667-plasmid-map-sequence-id-257080

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  20. Gr4-T2A-QF2 HDR plasmid

    Plasmid
    #140944
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140944-plasmid-map-sequence-id-313602

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Showing: 81 - 100 of 49183 results