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Plasmid#154350PurposeMammalian expression of MTnDepositorInsertMTn
UseTagsAcGFPExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10
Plasmid#181859PurposePart of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells.DepositorInsertcp-PKN-mRuby2-GFP(1-0)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5AdeltaSIV GFP Nter
Plasmid#145770Purposeexpresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
UseTagsGFPExpressionMammalianMutationStop codon at Serine 2013PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2
Plasmid#112837Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH2 + SH3 (C-term) domains of Grb2PromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Ost4-GFP-MTn
Plasmid#154351PurposeMammalian expression of MTnDepositorInsertMTn
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP-Flag-UTR
Plasmid#183235PurposeTo express FLAG tagged eGFPDepositorInsertEGFP
UseTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CIM5 (Rpt-1) GFP
Plasmid#16074DepositorInsertCIM5 (RPT1 Budding Yeast)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EGFP(W58stop)-HiBiT
Plasmid#180508PurposeMammalian expression of EGFP(W58stop)-HiBiTDepositorInsertEGFP(W58stop)
UseTagsHiBiTExpressionMammalianMutationW58(UGG) to amber stop codon(UAG)PromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP-MTn-KDEL
Plasmid#154348PurposeMammalian expression of MTnDepositorInsertMTn
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP Rab11a WT pcDNA3.1 Zeo (+)
Plasmid#206079PurposeRab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomesDepositorInsertRAB11A (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RIαΔ(D/D+linker)-EGFP
Plasmid#181843PurposeGFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationDeletion of residues 12-112 in PKA RIα.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP-2AFP-KDEL
Plasmid#154349PurposeMammalian expression of 2AFPDepositorInsert2AFP
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-LL5BIP
Plasmid#112829Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-mEGFP-pp4640
Plasmid#191846PurposeExpresses Salmon Alphavirus polyprotein with mEGFP N-terminal fusionDepositorInsertmEGFP-pp4640
UseTagsmEGFPExpressionMammalianMutationmEGFP fusion in Nterminal, some silent point muta…PromoterCMVAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5
Plasmid#112840Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationFull length Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-GFP
Plasmid#50486Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsGFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the GFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα(D/D+linker)-EGFP
Plasmid#181842PurposeEGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationContains only residues 12-112 of PKA RIα.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4
Plasmid#112839Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH3 (N-term) domain of Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4
Plasmid#112844Purposetransient/stable overexpression of the N-terminal and central domains of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationN + C1 domains of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2
Plasmid#112842Purposetransient/stable overexpression of the central domain of Catulin.DepositorInsertCatulin-alpha-1 (Ctnnal1 Mouse)
UseTagsGFPExpressionMammalianMutationC1 domain of CatulinPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only