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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85006

Purpose

For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

Plasmid

#179585

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NLS-NG-T2A

Expression

Mutation

Promoter

CAGp

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

Plasmid

#85179

Purpose

For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) tagRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Expression

Mutation

Promoter

TRE

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Use

Tags

ALFA tag

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Plasmid

#217511

Purpose

KD Recombinase dependent conditional smGdP-10XHA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA

Use

Tags

smGdP-10XHA

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Plasmid

#217510

Purpose

KD Recombinase dependent conditional smGdP-10XV5 cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Use

Tags

smGdP-10XV5

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Use

Tags

smGdP-10XOllas

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

Plasmid

#197044

Purpose

In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

Depositor

Liqun Luo

Article

Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

Inserts

pCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA

Use

Tags

HA, myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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loxP-T2A-Myr-DeadCasp8-ERT2-rox-FNF-loxP-T2A-Myr-Casp8-ERT2-rox

Plasmid

#182360

Purpose

Shuttle vector for T2A-Myr-DeadCasp8-ERT2 and T2A-Myr-Casp8-ERT2

Depositor

Mario Capecchi

Insert

Myr-DeadCasp8-ERT2 and Myr-Casp8-ERT2

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

Plasmid

#172480

Purpose

H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Article

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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ChrII_ttTi5605_loxP_myo2_neoR

Plasmid

#194056

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ttTi5605

Use

Tags

Expression

Worm

Mutation

Promoter

None

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ChrV_oxTi365_loxP_myo2_neoR

Plasmid

#194057

Purpose

Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

oxTi365

Use

Tags

Expression

Worm

Mutation

Promoter

None

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti.CAG.H2B-cerFP.W.SINloxP

Plasmid

#51006

Purpose

Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

Depositor

Rusty Lansford

Insert

H2B-cerFP

Use

Lentiviral

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLOXp-GUS

Plasmid

#154059

Purpose

Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

beta-glucoronidase (E. coli)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEXSISERS_FLuc_loxP-PuroR

Plasmid

#177866

Purpose

Cloning Backbone for Firefly-luciferase-based EXSISERS containing loxP-sites flanked PuroR cassette

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

Insert

FLuc-based EXSISERS

Use

CRISPR, Cre/Lox, Luciferase, Synthetic Biology, a…

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX330.LoxPO

Plasmid

#66585

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pAV10.F6.loxP

Plasmid

#63201

Purpose

pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 13, 2019

Availability

Academic Institutions and Nonprofits only

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