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Plasmid#101854PurposeGolden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheTagsExpressionMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only
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pNIC-OmpA-NHSTIIT-GG
Plasmid#215790PurposeCloning of target gene with N terminal OmpA SEC pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-SufI-NHSTIIT-GG
Plasmid#215791PurposeCloning of target gene with N terminal SufI TatA pathway signal peptide His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-SUMO1-AVITAG-GG
Plasmid#215808PurposeCloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pND005
Plasmid#200951PurposeYeast two-hybrid destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast two-hybridTagsExpressionMutationPromoterGAL4Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
UseTags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG005
Plasmid#113980PurposeLevel 1 high copy number vector for E. coli expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pND003
Plasmid#200949PurposeYeast surface display destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseYeast surface displayTagsAGA2, HA, and MycExpressionYeastMutationPromoterGAL1Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB1532S-RFP
Plasmid#101856PurposeGolden-Gate compatible fungal transformation vector carrying Sulfonylurea Resistance (Chlorimuron Ethyl) with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)DepositorArticleTypeEmpty backboneUseFungal expression plasmid, tested in magnaportheTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOGG041
Plasmid#113997PurposeLevel 0 module. Taurine-inducible promoter for AlphaproteobacteriaDepositorInsertTaurine promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pND004
Plasmid#200950PurposeMBP-tagged E. coli protein expression destination vector for Golden Gate cloningDepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC
Plasmid#170772Purposetest plasmid for Golden Gate assembly, expresses lacI repressorDepositorInsertlacI
UseTagsExpressionMutationPromoterlacI promoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOGG001
Plasmid#113978PurposeLevel 0 module. nptII promoter for constitutive gene expressionDepositorInsertnptII promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG037
Plasmid#113995PurposeSC module. Super-folder GFP green fluorescence reporterDepositorInsertSuper-folder GFP
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG050
Plasmid#113999PurposeSC module. Thermostable beta-galactosidase reporterDepositorInsertcelB
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG031
Plasmid#113994PurposeLevel 0 module. IPTG promoter for inducible expressionDepositorInsertIPTG-inducible promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG030
Plasmid#113993PurposeLevel 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purificationDepositorInsertIPTG-inducible T7RNAP promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG082
Plasmid#114000PurposeLevel 0 module. NifH promoter for nodule-specific expressionDepositorInsertNifH promoter
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSGV Hu Acceptor
Plasmid#64269PurposeRetroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genesDepositorTypeEmpty backboneUseRetroviralTagsnoneExpressionMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only