Skip to main content
Addgene
Showing: 81 - 93 of 93 results

  1. pSKAP_AO

    Plasmid
    #122717
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AO" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AO
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122717-plasmid-map-sequence-id-244412

    Enlarge

  2. pSKAP_AI

    Plasmid
    #122711
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AI" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122711-plasmid-map-sequence-id-244411

    Enlarge

  3. pSKAP_AH

    Plasmid
    #122710
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AH" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AH
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    122710-plasmid-map-sequence-id-244098

    Enlarge

  4. pSKAP_AG

    Plasmid
    #122709
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AG" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification.
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AG
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    122709-plasmid-map-sequence-id-244097

    Enlarge

  5. pSKAP_AE

    Plasmid
    #122707
    Purpose
    pSKAP plasmid containing the unique DNA barcode "AE" to be used for allelic exchange on bacterial chromosomes and subsequent molecular identification
    Depositor
    Travis Bourret
    Article
    Shaw et al J Vis Exp. 2019 May 13;(147). doi: 10.37
    Insert
    Barcode AE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    122707-plasmid-map-sequence-id-244094

    Enlarge

  6. pCACTUS

    Plasmid
    #167559
    Purpose
    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.
    Depositor
    Renato Morona
    Article
    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do
    Type
    Empty backbone
    Use
    Suicide cloning vector, temperature sensitive.
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    167559-plasmid-map-sequence-id-332373

    Enlarge

  9. pAX2

    Plasmid
    #117398
    Purpose
    allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch
    Depositor
    Karen Guillemin
    Article
    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    117398-plasmid-map-sequence-id-230535

    Enlarge

  10. pMSR

    Plasmid
    #161983
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161983-plasmid-map-sequence-id-321920

    Enlarge

  11. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Anat Herskovits
    Article
    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98783-plasmid-map-sequence-id-189220

    Enlarge

  12. pMSR0

    Plasmid
    #161984
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161984-plasmid-map-sequence-id-321926

    Enlarge

  13. pAOJ15

    Plasmid
    #91567
    Purpose
    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline promoter
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    91567-plasmid-map-sequence-id-181626

    Enlarge

Showing: 81 - 93 of 93 results