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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Use

Tags

6xHis-TEV

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2

Plasmid

#190898

Purpose

Entry vector for multiple sgRNA cloning

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6 and 7sk

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Use

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Mutation

Promoter

ptac

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ8702 pHR-TRE3G-AcrVA1-EF1a-sfGFP-T2A-rtta-WPRE

Plasmid

#140229

Purpose

Dox-inducible expression of anti-Cpf1 AcrVA1. Constitutive EF1a driven rtTA and GFP

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

AntiCRISPR protein AcrVA1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-CASANOVA

Plasmid

#113035

Purpose

CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CASANOVA

Plasmid

#113036

Purpose

AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS1026-pMCSG7-HpaCas9

Plasmid

#121540

Purpose

Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

HpaCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS1027-pMCSG7-SmuCas9

Plasmid

#121541

Purpose

Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

SmuCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

Plasmid

#123657

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

mCherry-P2A-DD-AcrIIA4

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

mCherry-P2A

Expression

Mammalian

Mutation

Promoter

CMV and mU6

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJH376

Plasmid

#86842

Purpose

mamalian expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH373

Plasmid

#86840

Purpose

mamalian expression of anti-CRISPR protein AcrIIA2

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSW21

Plasmid

#86836

Purpose

bacterial expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRAU171

Plasmid

#86835

Purpose

bacterial expression of anti-CRISPR protein AcrIIA2

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA2

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH375

Plasmid

#86841

Purpose

mamalian expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAU168

Plasmid

#86834

Purpose

bacterial expression of anti-CRISPR protein AcrIIA1

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA1

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSW24

Plasmid

#86837

Purpose

bacterial expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSW65

Plasmid

#86838

Purpose

bacterial expression of anti-CRISPR protein AcrIIA3

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA3

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

proC and tracrRNA promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge