Showing: 81 - 100 of 204 results
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Plasmid#159954Purposeuntargeted markerDepositorInsertYPET-C1
UseAdenoviralTagsExpressionMammalianMutationsyntheticPromoterhSyn1AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-NES
Plasmid#138203PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub(T/A)-YPet-NES
Plasmid#138205PurposeEncodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-Kras
Plasmid#138206PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub-YPet-NES
Plasmid#138207PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymYPET-T2A-mTurquoise2
Plasmid#118444PurposeProduces equimolar expression of ymYPET and mTq2DepositorInsertymYPET-T2A-mTurquoise2
UseTagsExpressionYeastMutationYPET A206K, F208S, E232L, N235DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW YPET-T2A-mTurquoise2
Plasmid#118450PurposeProduces equimolar expression of YPET and mTq2DepositorInsertYPET-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsubDD-YPet-NES
Plasmid#84633PurposeEncodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsubDD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-NES
Plasmid#84635PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)
Plasmid#127636PurposeEncodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)DepositorInsertmTurquoiuse2-Tat, YPet-PCP
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymYPET-URA3
Plasmid#168056PurposeReplaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymYPET
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFOX8-Tier1-PhCMV-YPet
Plasmid#169506PurposeTier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).DepositorInsertPCMV-driven yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailabilityAcademic Institutions and Nonprofits only -
pVH230-2-Tier1-PmPGK1-YPet
Plasmid#169521PurposeTier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).DepositorInsertPPGK-driven yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPPGKAvailabilityAcademic Institutions and Nonprofits only -
pTS2388-Tier1-PhCMV-p2a_Ypet
Plasmid#169525PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).DepositorInsertPCMV-driven P2A-fused yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailabilityAcademic Institutions and Nonprofits only -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 204 results