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Showing: 81 - 100 of 7036 results

  3. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid
    #41863
    Purpose
    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Insert
    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41863-plasmid-map-sequence-id-57052

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  6. Kv1.4 K+ channel (extracellular) scFv [L71/5]

    Plasmid
    #190517
    Purpose
    Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)
    Use
    Tags
    HA, Sortase, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    190517-plasmid-map-sequence-id-377123

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  7. Anti-Kv1.4 K+ channel (extracellular) [L71/22]

    Plasmid
    #190291
    Purpose
    Mammalian Expression Plasmid of anti-Kv1.4 K+ channel (extracellular) (Rat). Derived from hybridoma L71/22.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant Mouse monoclonal antibody (Kcna4 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190291-plasmid-map-sequence-id-376367

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  8. Anti-Kv1.4 K+ channel (extracellular) [L71/5R]

    Plasmid
    #177488
    Purpose
    Mammalian Expression Plasmid of anti-Kv1.4 K+ channel (extracellular) (Rat). Derived from hybridoma L71/5.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcna4 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177488-plasmid-map-sequence-id-348785

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  9. Anti-Pan-Neurofascin (extracellular) [A12/18R]

    Plasmid
    #177438
    Purpose
    Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177438-plasmid-map-sequence-id-348771

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  10. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    Erik Sontheimer, James Thomson
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

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  17. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138525-plasmid-map-sequence-id-292292

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  18. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid
    #138524
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    PRC2-binding G-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138524-plasmid-map-sequence-id-292291

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  19. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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Showing: 81 - 100 of 7036 results