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Plasmid#206138PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA (Acta2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-Neo-EF1a-GFP
Plasmid#69134PurposeSleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promotersDepositorTypeEmpty backboneUseSleeping beauty transposonTagsExpressionMammalianMutationPromoterU3MSCVAvailable sinceJan. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB_PhiC31LandingSite
Plasmid#48875PurposeSleeping beauty transposon with the PhiC31 landing site in a cmlc2::EGFP selection cassetteDepositorInsertcmlc2::attP-EGFP
UseTagsExpressionMutationPromoterAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTS395_PhCMV-SB100
Plasmid#169633PurposeExpression vector encoding PhCMV driven SB100 sleeping beauty transposase (PhCMV-SB100-pA)DepositorInsertPCMV-driven SB100
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-mAID/Zeo
Plasmid#171681PurposeTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)DepositorTypeEmpty backboneUseTagsmAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-mAID/Bla
Plasmid#171680PurposeTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)DepositorTypeEmpty backboneUseTagsmAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-AID/Bla
Plasmid#171678PurposeTRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (blasticidin)DepositorTypeEmpty backboneUseTagsAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-AID/Hyg
Plasmid#171677PurposeTRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin)DepositorTypeEmpty backboneUseTagsAIDExpressionMammalianMutationPromoterTREAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS1107_Tier3(SB)
Plasmid#169634PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase (5'ITR-A1-pA::A2-pA::A3-pA-3'ITR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.Z
Plasmid#79470PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, ZeocinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.IP
Plasmid#79472PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, IRES PuromycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.B
Plasmid#79460PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selectionDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.N
Plasmid#79468PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, NeomycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1/Pur
Plasmid#171682Purposeconstitutive expression of TIR1-myc in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertOsTIR1-MYC
UseTagsMYCExpressionMammalianMutationPromoterEF-1a promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.H
Plasmid#79464PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selectionDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Neo
Plasmid#171684Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)DepositorInsertNeomycin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUHD-SB-mAID/Hyg
Plasmid#171679PurposeTRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (hygromycin)DepositorInsertmAID
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Zeo
Plasmid#171685Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)DepositorInsertZeocin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat
Plasmid#133779PurposeExpresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (IDH1 Human)
UseTagsMyc and FLAGExpressionMammalianMutationArginine is mutated to histidine at amino acid po…PromoterAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only