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Plasmid#11534DepositorInsertIscU
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAX1_Z11 gpN:SpyTag
Plasmid#225196PurposeUsed for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher003:mNG
Plasmid#225190PurposeUsed for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 siteDepositorInsertSpyCatcher003:mNeonGreen
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-P-Domain
Plasmid#201193PurposeExpresses furin P-domain genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-P-Domain
UseTagsHisTag and SpyCatcher001ExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2796 (pSpy0C4)
Plasmid#220280Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA-GFP
Plasmid#220189PurposesgRNA expression vector - pEM7 promoter with GFP in sgRNA siteDepositorInsertpEM7 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyB-GFP
Plasmid#220190PurposesgRNA expression vector - pBG35 promoter with GFP in sgRNA siteDepositorInsertpBG35 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG35Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD-GFP
Plasmid#220192PurposesgRNA expression vector - pBG28 promoter with GFP in sgRNA siteDepositorInsertpBG28 promoter with GFP in sgRNA site
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Quartet-SpyTag
Plasmid#214726PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag fusionDepositorInsertQuartet-SpyTag (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet
Plasmid#214727PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet_NoLinker
Plasmid#214731PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTagDepositorInsertSpyTag-Quartet_NoLinker (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP786_SpyCatcher-EZH2-FL-CO
Plasmid#211779PurposeSpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-KRAB
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-DIIG
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
NDV-HN-SpyTag
Plasmid#160000PurposeExpresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cellsDepositorInsertNDV-HN-SpyTag
UseTagsHis6-tagExpressionMammalianMutationPromoterChicken beta-actin promoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B4.SPy1128
Plasmid#11531DepositorInsertphosphotransacetylase
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cys-3HB-SPYcatcher
Plasmid#214838PurposeThis plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertCys-3HB-SPYcatcher
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only